Wgląd w specyficzność wiązania peptydów na końcu C domeny PDZ neuronalnej syntazy tlenku azotu
Neuronowa syntaza tlenku azotu, w przeciwieństwie do jej śródbłonkowych i indukowalnych odpowiedników, wykazuje domenę PDZ (PSD-95/Dlg/ZO-1) zlokalizowaną na jej końcu N zaangażowaną w celowanie subkomórkowe. C-końce różnych białek komórkowych wstawiają się w bruzdę wiążącą tej domeny PDZ i określają subkomórkową dystrybucję neuronalnego NOS (nNOS). Mechanizmy molekularne leżące u podstaw tych interakcji są słabo poznane, ponieważ domena PDZ nNOS może najwyraźniej wykazywać specyficzność wiązania klasy I, klasy II i klasy III.
Ponadto ostatnio zasugerowano, że domena PDZ nNOS wiąże się z bardzo niskim powinowactwem z C-końcami białek docelowych i aby doszło do wiązania, musi zajść konieczna równoczesna interakcja boczna. Opisujemy tutaj, że domena PDZ nNOS może zachowywać się jak domena PDZ klasy III w dobrej wierze i wiązać się z sekwencjami C-końcowymi z resztami kwasowymi w pozycji P-2 z niskimi mikromolowymi stałymi wiązaniami. Wiązanie z sekwencjami C-końcowymi z resztą hydrofobową w pozycji P-2 oraz resztą kwasową w pozycji P-3 (klasa II) może również wystąpić, chociaż interakcje obejmujące reszty rozciągające się do pozycji P-7 pośredniczą w tego typu wiążący.
To rozwiązłe zachowanie rozciąga się również na jego powiązanie z sekwencjami klasy I, które muszą wykazywać resztę Glu w P-3 i resztę Thr w P-2 Za pomocą ukierunkowanej mutagenezy i spektroskopii NMR byliśmy w stanie zidentyfikować reszty zaangażowanych w każdy konkretny rodzaj wiązania i zracjonalizować mechanizmy stosowane do rozpoznawania partnerów wiążących.
Na koniec przeanalizowaliśmy związek o wysokim powinowactwie domeny PDZ nNOS z klaudyną-3 i klaudyną-14, dwoma ściśle połączonymi białkami błonowymi tetraspanowymi, które są niezbędnymi składnikami bariery parakomórkowej.
Wcześniejsze badania wykazały, że c-jun i neuronalna syntaza tlenku azotu (nNOS) są indukowane w uszkodzonych neuronach ruchowych, ale ich rola w śmierci neuronów ruchowych pozostaje niejasna.
- Postawiliśmy hipotezę, że nNOS może być dalszym efektorem kinazy N-końcowej c-jun (JNK)/c-jun w śmierci neuronu ruchowego wywołanej oderwaniem. Tutaj odkryliśmy, że oderwanie korzenia ramiennego wywołało przejściowy wzrost aktywności JNK i odpowiednio trzy- i czterokrotny wzrost fosfo-c-jun i c-jun; jednak oderwanie korzenia ramiennego spowodowało zmniejszenie ekspresji białka nNOS od 4 godzin do 14 dni po urazie.
- W 14 dni po urazie prawie wszystkie neurony ruchowe nNOS-dodatnie były zlokalizowane razem z neuronami ruchowymi fosfo-c-jun-dodatnimi w ipsilateralnych rogach brzusznych.
- Inhibitor JNK SP600125, zastosowany bezpośrednio po urazie, powodował podwyższenie poziomu białka nNOS zarówno w uszkodzonych rdzeniach kręgowych, jak i neuronach ruchowych i powodował niewielkie złagodzenie śmierci neuronów ruchowych poprzez hamowanie fosforylacji c-jun 14 dni po urazie.
- Nasze wyniki wykazały, że szlak transdukcji sygnału JNK/c-jun jest zaangażowany w oderwanie korzenia. Hamowanie fosforylacji c-jun zapobiega spadkowi poziomów nNOS poniżej poziomu wyjściowego w rdzeniu kręgowym i częściowo łagodzi śmierć neuronu ruchowego po wyrwaniu korzenia.
- W związku z tym hamowanie fosforylacji c-jun lub zwiększanie poziomu białka nNOS w uszkodzonym rdzeniu kręgowym na wczesnym etapie może zostać wykorzystane w przyszłości jako strategie celów molekularnych w zapobieganiu degeneracji neuronów ruchowych podczas wybijania korzeni.
Ogon C-końcowy (CT) neuronalnej syntazy tlenku azotu (nNOS) jest elementem regulatorowym, który hamuje aktywność nNOS przy braku związanej kalmoduliny (CaM).
Struktura krystaliczna domeny reduktazy nNOS (nNOSr) (Garcin, ED, Bruns, CM, Lloyd, SJ, Hosfield, DJ, Tiso, M., Gachhui, R., Stuehr, DJ, Tainer, JA i Getzoff, ED (2004) J. Biol. Chem. 279, 37918-37927) ujawnili, w jaki sposób pierwsza połowa CT oddziałuje z nNOSr, a zatem dostarczyli szablonu do szczegółowych badań. Wygenerowaliśmy mutanty skrócone w nNOS i nNOSr, aby przetestować znaczenie 3 różnych regionów CT.
Wyeliminowanie końcowej połowy CT (wszystkich reszt od Ile1413 do Ser1429), która jest niewidoczna w strukturze kryształu, nie miało prawie żadnego wpływu na uwalnianie NADP+, redukcję flawiny, autooksydację flawiny, redukcję hemu, aktywność reduktazy lub aktywność syntezy NO, ale zapobiegał wzrostowi ekranowania FMN, który normalnie występuje w odpowiedzi na wiązanie NADPH.
Dodatkowe usunięcie alfa-helisy CT (reszty 1401 do 1412) znacząco zwiększyło szybkość uwalniania NADP+, autooksydację flawiny i aktywność oksydazy NADPH oraz spowodowało nadmierne odsłanianie kofaktora FMN. Efekty te były związane ze zwiększoną aktywnością reduktazy i nieznacznie zmniejszoną redukcją hemu i syntezą NO.
Dalsze usuwanie reszt za Gly1396 (pełne skrócenie CT) wzmocniło wyżej wymienione efekty, a ponadto zmieniło oddziaływanie NADP+ z FAD, złagodziło kinetyczną supresję redukcji flawiny i dalej zmniejszyło redukcję hemu i syntezę NO. Nasze wyniki pokazują, że CT wywiera zarówno wieloaspektowy, jak i regiospecyficzny wpływ na aktywność katalityczną i powiązane zachowania, a tym samym dostarcza nowego wglądu w mechanizmy regulujące katalizę nNOS.
Domeny PDZ to wielofunkcyjne motywy interakcji białek, które często wiążą się z C-końcem docelowych białek. Tlenek azotu (NO), endogenna cząsteczka sygnałowa, odgrywa kluczową rolę w funkcjonowaniu układu nerwowego, odpornościowego i sercowo-naczyniowego. Chociaż istnieje wiele funkcji fizjologicznych dla pochodzącego z neuronów NO, wytwarzanego głównie przez neuronalną syntazę NO (nNOS), nadmierna aktywność nNOS pośredniczy w uszkodzeniu mózgu w niedokrwieniu mózgu i w zwierzęcych modelach choroby Parkinsona.
Goat Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A47504 | BlueGene | 96T | 700 EUR |
Rat Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E02C2363-192T | BlueGene | 192 tests | 1524 EUR |
Rat Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E02C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Rat Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E02C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E07C2363-192T | BlueGene | 192 tests | 1524 EUR |
Pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E07C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E07C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Goat Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E06C2363-192T | BlueGene | 192 tests | 1524 EUR |
Goat Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E06C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Goat Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E06C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Dog Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E08C2363-192T | BlueGene | 192 tests | 1524 EUR |
Dog Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E08C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Dog Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E08C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Rat Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A12592 | BlueGene | 96T | 700 EUR |
Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A21333 | BlueGene | 96T | 700 EUR |
Canine Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A64936 | BlueGene | 96T | 700 EUR |
Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A73650 | BlueGene | 96T | 700 EUR |
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A3831 | BlueGene | 96T | 700 EUR |
Rabbit Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A30071 | BlueGene | 96T | 700 EUR |
Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E03C2363-192T | BlueGene | 192 tests | 1524 EUR |
Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E03C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Mouse Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E03C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Bovine Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A82369 | BlueGene | 96T | 700 EUR |
Rabbit Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E04C2363-192T | BlueGene | 192 tests | 1524 EUR |
Rabbit Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E04C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Rabbit Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E04C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Sheep Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A99798 | BlueGene | 96T | 700 EUR |
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01C2363-192T | BlueGene | 192 tests | 1524 EUR |
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
|||
| E01C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
|||
| E01C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E09C2363-192T | BlueGene | 192 tests | 1524 EUR |
Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
|||
| E09C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Monkey Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
|||
| E09C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein (NOS1AP) ELISA Kit |
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| MBS7220453-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 5685 EUR |
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein (NOS1AP) ELISA Kit |
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| MBS7220453-48StripWells | MyBiosource | 48-Strip-Wells | 485 EUR |
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein (NOS1AP) ELISA Kit |
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| MBS7220453-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3020 EUR |
Human Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein (NOS1AP) ELISA Kit |
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| MBS7220453-96StripWells | MyBiosource | 96-Strip-Wells | 690 EUR |
Porcine Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A56221 | BlueGene | 96T | 700 EUR |
Chicken Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A91092 | BlueGene | 96T | 700 EUR |
Guinea Pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E01A38786 | BlueGene | 96T | 700 EUR |
Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
|||
| E05C2363-192T | BlueGene | 192 tests | 1524 EUR |
Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
|||
| E05C2363-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Guinea pig Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein(NOS1AP) ELISA kit |
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| E05C2363-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
nNOS (Neuronal Nitric Oxide Synthase) |
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| RA19011 | Neuromics | 100 uL | 451.2 EUR |
nNOS (Neuronal Nitric Oxide Synthase) |
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| MBS6508020-005mL | MyBiosource | 0.05mL | 860 EUR |
nNOS (Neuronal Nitric Oxide Synthase) |
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| MBS6508020-5x005mL | MyBiosource | 5x0.05mL | 3725 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (AP) |
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| MBS6452983-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (AP) |
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| MBS6452983-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (PE) |
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| MBS6452993-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (PE) |
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| MBS6452993-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (AP) |
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| MBS6257995-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (AP) |
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| MBS6257995-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase)-PE |
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| MBS6259037-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase)-PE |
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| MBS6259037-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (APC) |
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| MBS6452984-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (APC) |
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| MBS6452984-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (HRP) |
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| MBS6452987-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (HRP) |
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| MBS6452987-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (APC) |
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| MBS6258203-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (APC) |
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| MBS6258203-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (HRP) |
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| MBS6258828-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (HRP) |
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| MBS6258828-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (FITC) |
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| MBS6452986-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (FITC) |
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| MBS6452986-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (FITC) |
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| MBS6258620-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (FITC) |
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| MBS6258620-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (Biotin) |
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| MBS6452985-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (Biotin) |
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| MBS6452985-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (Biotin) |
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| MBS6258412-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (Biotin) |
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| MBS6258412-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
Neuronal Nitric Oxide Synthase (Nnos) Antibody |
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| 20-abx114082 | Abbexa |
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nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 405) |
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| MBS6452988-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 405) |
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| MBS6452988-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 490) |
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| MBS6452989-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 490) |
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| MBS6452989-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 550) |
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| MBS6452990-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 550) |
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| MBS6452990-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 650) |
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| MBS6452991-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 650) |
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| MBS6452991-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 750) |
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| MBS6452992-01mL | MyBiosource | 0.1mL | 895 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 750) |
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| MBS6452992-5x01mL | MyBiosource | 5x0.1mL | 3875 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 405) |
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| MBS6259246-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 405) |
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| MBS6259246-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 490) |
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| MBS6259455-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 490) |
|||
| MBS6259455-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 550) |
|||
| MBS6259664-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 550) |
|||
| MBS6259664-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 650) |
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| MBS6259873-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 650) |
|||
| MBS6259873-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 750) |
|||
| MBS6260082-01mL | MyBiosource | 0.1mL | 1055 EUR |
nNOS (Neuronal Nitric Oxide Synthase) (MaxLight 750) |
|||
| MBS6260082-5x01mL | MyBiosource | 5x0.1mL | 4585 EUR |
nNOS-C-terminus (Neuronal Nitric Oxide Synthase) |
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| P24287 | Neuromics | 50 ug Blocking Peptide | 208.8 EUR |
nNOS-C-terminus (Neuronal Nitric Oxide Synthase) |
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| RA24287 | Neuromics | 100 uL | 646.8 EUR |
nNOS-C-terminus (Neuronal Nitric Oxide Synthase) |
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| RA24287-B | Neuromics | each | 371.25 EUR |
nNOS-C-terminus (Neuronal Nitric Oxide Synthase) |
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| MBS556173-005mg | MyBiosource | 0.05mg | 220 EUR |
nNOS-C-terminus (Neuronal Nitric Oxide Synthase) |
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| MBS556173-5x005mg | MyBiosource | 5x0.05mg | 845 EUR |
Rat neuronal nitric oxide synthase(nNOS) Elisa Kit |
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| EK720992 | AFG Bioscience LLC | 96 Wells | 0.62 EUR |
Rat neuronal nitric oxide synthase,nNOS ELISA Kit |
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| YLA0034RA-48T | Shanghai YL Biotech | 48T | Ask for price |
Rat neuronal nitric oxide synthase,nNOS ELISA Kit |
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| YLA0034RA-96T | Shanghai YL Biotech | 96T | Ask for price |
Rat neuronal nitric oxide synthase(nNOS)ELISA Kit |
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| SL1065Ra | Sunlong | - | 498 EUR |
Mouse neuronal nitric oxide synthase(nNOS) Elisa Kit |
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| EK730436 | AFG Bioscience LLC | 96 Wells | 0.68 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
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| EKC34795-48T | Biomatik Corporation | 48T | 474.04 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
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| EKC34795-5x96T | Biomatik Corporation | 5x96T | 3216.7 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
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| EKC34795-96T | Biomatik Corporation | 96T | 677.2 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) Elisa Kit |
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| EK713153 | AFG Bioscience LLC | 96 Wells | 0.33 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
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| CSB-E13872h-24T | Cusabio | 1 plate of 24 wells | 198 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
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| 1-CSB-E13872h | Cusabio |
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Human Neuronal Nitric Oxide Synthase,nNOS ELISA KIT |
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| E5761Hu-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | 4122 EUR |
Human Neuronal Nitric Oxide Synthase,nNOS ELISA KIT |
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| E5761Hu-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | 300 EUR |
Human Neuronal Nitric Oxide Synthase,nNOS ELISA KIT |
|||
| E5761Hu-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | 2061 EUR |
Human Neuronal Nitric Oxide Synthase,nNOS ELISA KIT |
|||
| E5761Hu-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | 458 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| RK01944 | Abclonal | 96 Tests | 280 EUR |
Mouse neuronal nitric oxide synthase(nNOS) ELISA Kit |
|||
| SL0689Mo | Sunlong | - | 528 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS700071-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 5325 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS700071-24StripWellsLIMIT1 | MyBiosource | 24-Strip-Wells(LIMIT1) | 275 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS700071-48StripWells | MyBiosource | 48-Strip-Wells | 480 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS700071-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 2795 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS700071-96StripWells | MyBiosource | 96-Strip-Wells | 675 EUR |
Plant Neuronal Nitric Oxide Synthase (NNOS) ELISA Kit |
|||
| MBS9374606-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS1606650-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 3460 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS1606650-48StripWells | MyBiosource | 48-Strip-Wells | 285 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS1606650-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 1750 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS ELISA Kit |
|||
| MBS1606650-96StripWells | MyBiosource | 96-Strip-Wells | 425 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS3801247-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS3801247-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS3801247-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Human Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS3801247-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
Canine Neuronal Nitric Oxide Synthase, NNOS ELISA KIT |
|||
| E0293Ca-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | 4275 EUR |
Canine Neuronal Nitric Oxide Synthase, NNOS ELISA KIT |
|||
| E0293Ca-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | 350 EUR |
Canine Neuronal Nitric Oxide Synthase, NNOS ELISA KIT |
|||
| E0293Ca-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | 2137 EUR |
Canine Neuronal Nitric Oxide Synthase, NNOS ELISA KIT |
|||
| E0293Ca-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | 475 EUR |
Canine Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS283382-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6840 EUR |
Canine Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS283382-48StripWells | MyBiosource | 48-Strip-Wells | 480 EUR |
Canine Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS283382-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3610 EUR |
Canine Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS283382-96StripWells | MyBiosource | 96-Strip-Wells | 805 EUR |
Monkey Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS285914-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6840 EUR |
Monkey Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS285914-48StripWells | MyBiosource | 48-Strip-Wells | 480 EUR |
Monkey Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS285914-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3610 EUR |
Monkey Neuronal Nitric Oxide Synthase (nNOS) ELISA Kit |
|||
| MBS285914-96StripWells | MyBiosource | 96-Strip-Wells | 805 EUR |
nNOS, aa1409-1429 (Neuronal Nitric Oxide Synthase, bNOS) |
|||
| MBS6011120-01mL | MyBiosource | 0.1(mL | 830 EUR |
nNOS, aa1409-1429 (Neuronal Nitric Oxide Synthase, bNOS) |
|||
| MBS6011120-5x01mL | MyBiosource | 5x0.1mL | 3595 EUR |
Human Neuronal Nitric Oxide Synthase, nNOS GENLISA ELISA |
|||
| KBH5761 | Krishgen | 1 x 96 wells | 286 EUR |
Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit |
|||
| GA-E0550RT-48Tests | GenAsia Biotech | 48 Tests | Ask for price |
Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit |
|||
| GA-E0550RT-96Tests | GenAsia Biotech | 96 Tests | 420 EUR |
Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit |
|||
| GA-E0550RT-48T | GenAsia Biotech | 48T | 380.4 EUR |
Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit |
|||
| GA-E0550RT-96T | GenAsia Biotech | 96T | 595.2 EUR |
Rat neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit |
|||
| QY-E11102 | Qayee Biotechnology | 96T | 433.2 EUR |
Rat neuronal nitric oxide synthase, NOS, nNOS ELISA Kit |
|||
| MBS164640-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 3955 EUR |
Rat neuronal nitric oxide synthase, NOS, nNOS ELISA Kit |
|||
| MBS164640-48StripWells | MyBiosource | 48-Strip-Wells | 305 EUR |
Rat neuronal nitric oxide synthase, NOS, nNOS ELISA Kit |
|||
| MBS164640-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 2005 EUR |
Rat neuronal nitric oxide synthase, NOS, nNOS ELISA Kit |
|||
| MBS164640-96StripWells | MyBiosource | 96-Strip-Wells | 475 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| EKC39476-48T | Biomatik Corporation | 48T | 474.04 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| EKC39476-5x96T | Biomatik Corporation | 5x96T | 3216.7 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| EKC39476-96T | Biomatik Corporation | 96T | 677.2 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| EK11198 | SAB | 96Т | 799 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| AE58201RA-48Tests | Abebio | 48 Tests | 360 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| AE58201RA-96Tests | Abebio | 96 Tests | 680 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| CSB-E14034r-24T | Cusabio | 1 plate of 24 wells | 198 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| 1-CSB-E14034r | Cusabio |
|
|
Rat neuronal nitric oxide synthase,NOS1;nNOS ELISA KIT |
|||
| E0542Ra-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | 4122 EUR |
Rat neuronal nitric oxide synthase,NOS1;nNOS ELISA KIT |
|||
| E0542Ra-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | 300 EUR |
Rat neuronal nitric oxide synthase,NOS1;nNOS ELISA KIT |
|||
| E0542Ra-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | 2061 EUR |
Rat neuronal nitric oxide synthase,NOS1;nNOS ELISA KIT |
|||
| E0542Ra-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | 458 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| AE58201RA-48T | Abebio | 48T | 360 EUR |
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| AE58201RA-96T | Abebio | 96T | 680 EUR |
Rat Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS704610-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 5325 EUR |
Rat Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS704610-24StripWellsLIMIT1 | MyBiosource | 24-Strip-Wells(LIMIT1) | 275 EUR |
Rat Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS704610-48StripWells | MyBiosource | 48-Strip-Wells | 480 EUR |
Rat Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS704610-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 2795 EUR |
Rat Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS704610-96StripWells | MyBiosource | 96-Strip-Wells | 675 EUR |
Mouse neuronal nitric oxide synthase(NOS,nNOS)ELISA Kit |
|||
| QY-E21072 | Qayee Biotechnology | 96T | 433.2 EUR |
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) Elisa Kit |
|||
| EK731356 | AFG Bioscience LLC | 96 Wells | 0.77 EUR |
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| CSB-E13273m-24T | Cusabio | 1 plate of 24 wells | 198 EUR |
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| 1-CSB-E13273m | Cusabio |
|
|
Mouse Neuronal Nitric Oxide Synthase,nNOS;NOS1 ELISA KIT |
|||
| E1803Mo-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | 4122 EUR |
Mouse Neuronal Nitric Oxide Synthase,nNOS;NOS1 ELISA KIT |
|||
| E1803Mo-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | 300 EUR |
Mouse Neuronal Nitric Oxide Synthase,nNOS;NOS1 ELISA KIT |
|||
| E1803Mo-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | 2061 EUR |
Mouse Neuronal Nitric Oxide Synthase,nNOS;NOS1 ELISA KIT |
|||
| E1803Mo-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | 458 EUR |
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| RK07085 | Abclonal | 96T | 280 EUR |
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| MBS1603280-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 3955 EUR |
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| MBS1603280-48StripWells | MyBiosource | 48-Strip-Wells | 305 EUR |
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| MBS1603280-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 2005 EUR |
Mouse Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| MBS1603280-96StripWells | MyBiosource | 96-Strip-Wells | 475 EUR |
Canine Neuronal Nitric Oxide Synthase (NOS, NNOS) ELISA Kit |
|||
| MBS1603356-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 4295 EUR |
Canine Neuronal Nitric Oxide Synthase (NOS, NNOS) ELISA Kit |
|||
| MBS1603356-48StripWells | MyBiosource | 48-Strip-Wells | 410 EUR |
Canine Neuronal Nitric Oxide Synthase (NOS, NNOS) ELISA Kit |
|||
| MBS1603356-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 2185 EUR |
Canine Neuronal Nitric Oxide Synthase (NOS, NNOS) ELISA Kit |
|||
| MBS1603356-96StripWells | MyBiosource | 96-Strip-Wells | 520 EUR |
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody |
|||
| 20-abx329607 | Abbexa |
|
|
Rat neuronal Nitric Oxide synthase, NOS,nNOS GENLISA ELISA |
|||
| KLR0542 | Krishgen | 1 x 96 wells | 341 EUR |
Bovine Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS9310041-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 6725 EUR |
Bovine Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS9310041-48StripWells | MyBiosource | 48-Strip-Wells | 550 EUR |
Bovine Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS9310041-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3420 EUR |
Bovine Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit |
|||
| MBS9310041-96StripWells | MyBiosource | 96-Strip-Wells | 765 EUR |
Chicken Neuronal Nitric Oxide Synthase(nNOS/NOS1)ELISA Kit |
|||
| YLA0003CH-48T | Shanghai YL Biotech | 48T | Ask for price |
Chicken Neuronal Nitric Oxide Synthase(nNOS/NOS1)ELISA Kit |
|||
| YLA0003CH-96T | Shanghai YL Biotech | 96T | Ask for price |
Chicken Neuronal Nitric Oxide Synthase,nNOS;NOS1 ELISA KIT |
|||
| E0156Ch-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | 4482 EUR |
Chicken Neuronal Nitric Oxide Synthase,nNOS;NOS1 ELISA KIT |
|||
| E0156Ch-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | 360 EUR |
Chicken Neuronal Nitric Oxide Synthase,nNOS;NOS1 ELISA KIT |
|||
| E0156Ch-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | 2241 EUR |
Chicken Neuronal Nitric Oxide Synthase,nNOS;NOS1 ELISA KIT |
|||
| E0156Ch-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | 498 EUR |
Chicken Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| MBS1601905-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 4470 EUR |
Chicken Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| MBS1601905-48StripWells | MyBiosource | 48-Strip-Wells | 415 EUR |
Chicken Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| MBS1601905-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 2270 EUR |
Chicken Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit |
|||
| MBS1601905-96StripWells | MyBiosource | 96-Strip-Wells | 540 EUR |
ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) |
|||
| KTE100825-48T | Abbkine | 48T | 424.8 EUR |
ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) |
|||
| KTE100825-5platesof96wells | Abbkine | 5 plates of 96 wells | 2702.4 EUR |
ELISA kit for Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) |
|||
| KTE100825-96T | Abbkine | 96T | 686.4 EUR |
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit |
|||
| abx595427-96tests | Abbexa | 96 tests | 764.4 EUR |
Mouse Neuronal Nitric Oxide Synthase, nNOS/NOS1 GENLISA ELISA |
|||
| KLM1803 | Krishgen | 1 x 96 wells | 341 EUR |
Canine Neuronal Nitric Oxide Synthase, NOS,NNOS GENLISA ELISA |
|||
| KLN0293 | Krishgen | 1 x 96 wells | 341 EUR |
Chicken Neuronal Nitric Oxide Synthase, nNOS/NOS1 GENLISA ELISA |
|||
| KLC0156 | Krishgen | 1 x 96 wells | 341 EUR |
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (HRP) |
|||
| 20-abx314989 | Abbexa |
|
|
Neuronal Nitric Oxide Synthase |
|||
| MBS472037-01mL | MyBiosource | 0.1mL | 370 EUR |
Neuronal Nitric Oxide Synthase |
|||
| MBS472037-5x01mL | MyBiosource | 5x0.1mL | 1505 EUR |
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (FITC) |
|||
| 20-abx314990 | Abbexa |
|
|
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit |
|||
| abx195276-96tests | Abbexa | 96 tests | 990 EUR |
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit |
|||
| 20-abx492066 | Abbexa |
|
|
Rat NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-R0746-24Tests | Elabscience Biotech | 24 Tests | 180 EUR |
Rat NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-R0746-48Tests | Elabscience Biotech | 48 Tests | 546 EUR |
Rat NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-R0746-96Tests | Elabscience Biotech | 96 Tests | 682 EUR |
Rat NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-R0746-96Tests10 | Elabscience Biotech | 96 Tests *10 | 6820 EUR |
Rat NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-R0746-96Tests5 | Elabscience Biotech | 96 Tests *5 | 3410 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit |
|||
| MBS2532333-5x96Tests | MyBiosource | 5x96Tests | 2565 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit |
|||
| MBS2532333-96Tests | MyBiosource | 96Tests | 560 EUR |
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit |
|||
| abx055223-96tests | Abbexa | 96 tests | 801.6 EUR |
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit |
|||
| abx256109-96tests | Abbexa | 96 tests | 801.6 EUR |
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit |
|||
| abx570103-96tests | Abbexa | 96 tests | 801.6 EUR |
Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit |
|||
| 20-abx155900 | Abbexa |
|
|
Pig Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit |
|||
| abx361104-96tests | Abbexa | 96 tests | 990 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| EKE62369-5x96T | Biomatik Corporation | 5x96T | 3124.08 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| EKE62369-96T | Biomatik Corporation | 96T | 657.7 EUR |
Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| ER0144 | FN Test | 96T | 571.5 EUR |
Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| RE1411R-48wells | Reed Biotech | 48 wells | 125.55 EUR |
Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| RE1411R-96wells | Reed Biotech | 96 wells | 179.55 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| MBS2507534-10x96Tests | MyBiosource | 10x96Tests | 4060 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| MBS2507534-24Tests | MyBiosource | 24Tests | 265 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| MBS2507534-48Tests | MyBiosource | 48Tests | 450 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| MBS2507534-5x96Tests | MyBiosource | 5x96Tests | 2265 EUR |
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| MBS2507534-96Tests | MyBiosource | 96Tests | 535 EUR |
Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| RE1411R-48wellsplate | Reed Biotech | 48-wells plate | 126 EUR |
Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| RE1411R-96wellsplate | Reed Biotech | 96-wells plate | 180 EUR |
Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| SYP-R0300-48wellsplate | UpingBio | 48 wells plate | 180 EUR |
Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit |
|||
| SYP-R0300-96wellsplate | UpingBio | 96 wells plate | 220 EUR |
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) Antibody (Biotin) |
|||
| 20-abx314991 | Abbexa |
|
|
Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit |
|||
| abx195274-96tests | Abbexa | 96 tests | 990 EUR |
Mouse Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) CLIA Kit |
|||
| abx195275-96tests | Abbexa | 96 tests | 990 EUR |
Mouse NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-M0697-24Tests | Elabscience Biotech | 24 Tests | 180 EUR |
Mouse NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-M0697-48Tests | Elabscience Biotech | 48 Tests | 546 EUR |
Mouse NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-M0697-96Tests | Elabscience Biotech | 96 Tests | 682 EUR |
Mouse NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-M0697-96Tests10 | Elabscience Biotech | 96 Tests *10 | 6820 EUR |
Mouse NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-M0697-96Tests5 | Elabscience Biotech | 96 Tests *5 | 3410 EUR |
Human NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-H0537-24Tests | Elabscience Biotech | 24 Tests | 180 EUR |
Human NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-H0537-48Tests | Elabscience Biotech | 48 Tests | 546 EUR |
Human NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-H0537-96Tests | Elabscience Biotech | 96 Tests | 682 EUR |
Human NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
|||
| E-CL-H0537-96Tests10 | Elabscience Biotech | 96 Tests *10 | 6820 EUR |
Human NOS1/nNOS -Nitric Oxide Synthase 1, Neuronal- CLIA Kit |
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| E-CL-H0537-96Tests5 | Elabscience Biotech | 96 Tests *5 | 3410 EUR |
Mouse NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit |
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| MBS2532674-5x96Tests | MyBiosource | 5x96Tests | 2565 EUR |
Mouse NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit |
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| MBS2532674-96Tests | MyBiosource | 96Tests | 560 EUR |
Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit |
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| MBS2532683-5x96Tests | MyBiosource | 5x96Tests | 2565 EUR |
Human NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) CLIA Kit |
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| MBS2532683-96Tests | MyBiosource | 96Tests | 560 EUR |
Human Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit |
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| 20-abx152535 | Abbexa |
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Dlatego też subkomórkowa lokalizacja aktywności nNOS musi być ściśle regulowana. Aby określić ligandy domeny PDZ nNOS, przeszukaliśmy 13 miliardów różnych peptydów i odkryliśmy, że domena nNOS-PDZ wiąże się ściśle z peptydami kończącymi się Asp-X-Val. Różni się to od jedynego znanego konsensusu (Thr/Ser)-X-Val, który oddziałuje z domenami PDZ z PSD-95. W preferencji dla Asp w pozycji peptydu -2 pośredniczy Tyr-77 z nNOS. Podstawienie Y77D78 do H77E78 zmienia specyficzność wiązania z Asp-X-Val na Thr-X-Val. Kierując się konsensusem Asp-X-Val, zidentyfikowano kandydujące białka oddziałujące z nNOS, w tym receptory glutaminianu i melatoniny. Nasze wyniki pokazują, że domeny PDZ mają wyraźną specyficzność wiązania peptydów.
