Aktywność przeciwdrobnoustrojowa nanocząsteczek
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Aktywność przeciwdrobnoustrojowa nanocząsteczek srebra z drzewa kamforowego przeciwko chorobom zgnilca i wykrycie nowego szczepu Serratia marcescens za pomocą DGGE-PCR jako wtórnego zakażenia larw pszczół miodnych
American foulbrood (AFB) and European foulbrood (EFB) are the 2 main bacterial ailments affecting honeybees, resulting in a lower in viability of the hive, lowering honey manufacturing, and leading to vital financial losses to beekeepers. As a result of inefficiency and/or low efficacy of some antibiotics, researches with nanotechnology symbolize, presumably, new therapeutic methods. Nanostructure medication have offered some advantagesover the standard medicines, akin to gradual, gradual and managed launch, elevated bioavailability, and diminished side-effects. On this examine, totally different contaminated larvae had been collected from two apiaries; the combs that had signs of American and European foulbrood had been remoted. In vitro antimicrobial exercise of camphor tree silver nano-particles in opposition to foulbrood ailments had been characterised utilizing UV-Vis spectrophotometry and scanning electron microscope (SEM) that proves the formation of silver nanoparticles with measurement vary 160-660 nm.
The antimicrobial exercise of the silver nanoparticles was examined utilizing agar diffusion assay and proved their potential to successfully stop the pathogenic bacterial progress in each AFB and EFB. DGGE-PCR method has been utilized for the identification of un-common bacterial infections honeybees relying on 16S rRNA amplification from their complete extracted DNA and has been recognized as Serratia marcescens (TES), deposited in GenBank with a brand new accession quantity (MT240613). The outcomes had been confirmed pressure has been detected by DGGE-PCR evaluation inflicting uniquely contaminated brood that was attacked by the American Foulbrood It could possibly be concluded that greenly synthesized silver nanoparticles is projected for use as efficient therapy for honeybee bacterial ailments. These materials want extra investigations below discipline circumstances and examine the opportunity of its residues in honeybee merchandise akin to honey, and beeswax.
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Przypadek nawrotu klinicznego SARS-CoV-2 po four negatywnych testach RT-PCR w Grecji: nawrót czy ponowna infekcja?
SARS-CoV-2 pandemic is the best public well being concern of the yr 2020. There are a number of worldwide experiences of sufferers who’ve managed to recuperate from SARS-CoV-2 an infection with detrimental PCR check outcomes, that for unknown causes convert again to optimistic PCR. We report a case of a affected person in our hospital who developed optimistic PCR check outcomes for SARS-CoV-2, after four consecutive outcomes that had been detrimental, together with a full-blown medical syndrome of SARS-CoV-2 an infection.
Ocena wydajności diagnostycznej dziewięciu komercyjnych zestawów RT-PCR do wykrywania SARS-CoV-2 w Kolumbii
The continuing SARS-CoV-2 pandemic has led to the design and growth of a number of RT-PCR kits aimed to facilitate the fast scale-up of molecular testing for enormous screening. We evaluated the diagnostic efficiency of 9 business kits, which confirmed optimum efficiency and excessive discriminatory energy. Nonetheless, we noticed variations by way of sensitivity, specificity, and E gene Ct Values and focus on these ends in mild of the affect of SARS-CoV-2 genetic variability and its potential impression in present molecular diagnostic assays. This text is protected by copyright. All rights reserved.
Jednoczesne wykrywanie i różnicowanie klinicznie istotnej nawracającej gorączki Borrelia z semi-multipleksowym PCR w czasie rzeczywistym
Bacterial vector-borne ailments, together with Borrelia species, current a big diagnostic, medical, and public well being problem on account of their overlapping signs and the breadth of causative brokers and arthropod vectors. The relapsing fever (RF) borreliae embody each established and rising pathogens and are transmitted to people by smooth ticks, exhausting ticks, or lice. We developed a real-time semi-multiplex PCR assay that detects a number of RF borreliae inflicting human sickness and classifies them into certainly one of three teams.
The teams are based mostly on genetic similarity and embody brokers of soft-tick relapsing fever (B. hermsii and others), the rising exhausting tick transmitted pathogen B. miyamotoi, and the agent of louse-borne relapsing fever (B. recurrentis) The actual-time PCR assay makes use of a single primer pair designed to amplify all identified pathogenic RF borreliae, and a number of TaqMan probes to permit for detection of and differentiation among the many three teams.
The assay detects all RF borreliae examined with an analytical restrict of detection under 15 genome equivalents per response. Thirty isolates of RF borreliae encompassing six species had been precisely recognized. Thirty-nine of 41 residual specimens (EDTA entire blood, serum, or plasma) from sufferers with RF had been detected and accurately categorized. None of 42 medical samples from sufferers with different infections and 46 tradition specimens from non-RF micro organism had been detected. The event of a single assay real-time PCR strategy will assist to enhance analysis of RF by simplifying the number of checks to help in medical administration of acutely unwell RF sufferers.
Analiza przeżycia czasu do negatywnej oceny PCR SARS-CoV-2 w celu optymalizacji recept PCR u pracowników służby zdrowia: badanie kohortowe pracowników służby zdrowia COVID-19 w Henares
Aims: Reverse transcriptase PCR (RT-PCR) is taken into account the gold customary in diagnosing COVID-19. Contaminated healthcare employees don’t return to work till RT-PCR has demonstrated that the virus is now not current within the higher respiratory tract. The purpose of this examine is to find out probably the most environment friendly time to carry out RT-PCR previous to healthcare employees’ reincorporation.
Supplies and strategies: This can be a cohort examine of healthcare employees with RT-PCR-confirmed COVID-19. Information had been collected utilizing the medical charts of healthcare employees and accomplished with a phone interview. Kaplan-Meier curves had been used to find out the affect of a number of variables on the time to RT-PCR negativisation. The impression of the variables on survival was assessed utilizing the Breslow check. A Cox regression mannequin was developed together with the related variables.
Outcomes: 159 topics with a optimistic RT-PCR out of 374 employees with suspected COVID-19 had been included. The median time to negativisation was 25 days from symptom onset (IQR 20-35 days). Presence of IgG, dyspnoea, cough and throat ache had been related to vital longer time to negativisation. Cox logistic regression was used to regulate for confounding variables. Solely dyspnoea and cough remained within the mannequin as vital determinants of extended negativisation time. Adjusted HRs had been 0.68 (0.48-096) for dyspnoea and 0.61 (0.42-0.88) for dry cough.
Conclusions: RT-PCR throughout the first three weeks results in a excessive share of optimistic outcomes. Within the presence of respiratory signs, negativisation took almost 1 week extra. Those that developed antibodies wanted longer time to negativisate.
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2 × EpiArt HS Taq Master Mix (Dye Plus) |
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EM202-01 | Vazyme | 1ml | EUR 16 |
2 × EpiArt HS Taq Master Mix (Dye Plus) |
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EM202-02 | Vazyme | 5 ml (5×1ml) | EUR 70 |
2 × EpiArt HS Taq Master Mix (Dye Plus) |
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EM202-03 | Vazyme | 15 ml (15×1ml) | EUR 192 |
2x Taq PCR Master Mix |
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A4145-1ML40Rxns | Biomatik Corporation | 1ML (40Rxns) | EUR 77 |
Description: Biotechnology |
Taq PCR Master Mix (2x) |
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E2520-01 | EURx | 100reactions | EUR 38.15 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs |
Taq PCR Master Mix (2x) |
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E2520-02 | EURx | 200reactions | EUR 71.94 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs |
Taq PCR Master Mix (2x) |
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E2520-03 | EURx | 500reactions | EUR 155.87 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs |
Taq 2X PCR Master Mix |
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RK20602 | Abclonal | 500RXN | EUR 12.54 |
Taq (exo-) 2X Master Mix |
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RK20612 | Abclonal | 100RXN | EUR 49.05 |
Taq Mix (2x) (PCR Master Mix (2x)) |
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50900 | Sisco Laboratories | 1 ml | EUR 21.38 |
Description: Part E |
Taq Mix (2x) (PCR Master Mix (2x)) |
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50900-1 | Sisco Laboratories | 5 x1 ml | EUR 64.14 |
Description: Part E |
Taq Mix (2x) (PCR Master Mix (2x)) |
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50900-2 | Sisco Laboratories | 5 x2.5 ml | EUR 128.29 |
Description: Part E |
2 × Taq Plus Master Mix |
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P211-01 | Vazyme | 5 ml | EUR 58.81 |
2 × Taq Plus Master Mix |
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P211-02 | Vazyme | 15 ml | EUR 162.24 |
2 × Taq Plus Master Mix |
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P211-03 | Vazyme | 50 ml | EUR 507 |
2 × Taq Plus Master Mix |
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P211-01-51ml | Vazyme | 5 × 1 ml | EUR 60.9 |
2 × Taq Plus Master Mix |
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P211-03-501ml | Vazyme | 50 × 1 ml | EUR 525 |
2X ViRed Taq Master Mix, 100app |
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CLMM01 | Vivantis | each | Ask for price |
ViPrimePLUS Taq qPCR Green Master Mix I with Low ROX, 150rxns |
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QLMM12-LR | Vivantis | each | Ask for price |
ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns |
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QLMM17-LR | Vivantis | each | Ask for price |
2 × Taq Plus Master Mix Ⅱ (Dye Plus) |
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P213-01 | Vazyme | 5 ml | EUR 58.81 |
2 × Taq Plus Master Mix Ⅱ (Dye Plus) |
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P213-02 | Vazyme | 15 ml | EUR 162.24 |
2 × Taq Plus Master Mix Ⅱ (Dye Plus) |
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P213-03 | Vazyme | 50 ml | EUR 507 |
2 × Taq Plus Master Mix II (Dye Plus) |
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P213-01-51ml | Vazyme | 5 × 1 ml | EUR 60.9 |
2 × Taq Plus Master Mix II (Dye Plus) |
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P213-02-151ml | Vazyme | 15 × 1 ml | EUR 168 |
2 × Taq Plus Master Mix II (Dye Plus) |
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P213-03-501ml | Vazyme | 50 × 1 ml | EUR 525 |
Taq 2X MeanGreen Master Mix |
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MBS308024-1000Reactions | MyBiosource | 1000Reactions | EUR 370 |
Taq 2X MeanGreen Master Mix |
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MBS308024-100Reactions | MyBiosource | 100Reactions | EUR 195 |
Taq 2X MeanGreen Master Mix |
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MBS308024-250Reactions | MyBiosource | 250Reactions | EUR 215 |
Taq 2X MeanGreen Master Mix |
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MBS308024-500Reactions | MyBiosource | 500Reactions | EUR 270 |
ViPrimePLUS OneStep Taq RT-qPCR Master Mix with Low Rox, 150rxns |
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QLMM13-LR | Vivantis | each | Ask for price |
2 × EpiArt HS Taq Master Mix |
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EM201-01 | Vazyme | 1ml | EUR 16 |
2 × EpiArt HS Taq Master Mix |
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EM201-02 | Vazyme | 5 ml (5×1ml) | EUR 70 |
4 × EpiArt HS Taq Master Mix |
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EM201-03 | Vazyme | 15 ml (15×1ml) | EUR 192 |
Apex 2X Taq Master Mix, Clear |
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42-133 | Genesee Scientific | 100 Reactions/Unit | EUR 54.78 |
Description: 1.5mM MgCl2 (Final Conc.) |
Apex 2X Taq Master Mix, Clear |
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42-134 | Genesee Scientific | 500 Reactions/Unit | EUR 220.95 |
Description: 1.5mM MgCl2 (Final Conc.) |
Apex 2X Taq Master Mix, Clear |
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42-134B | Genesee Scientific | 1000 Reactions/Unit | EUR 433.29 |
Description: 1.5mM MgCl2 (Final Conc.) |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
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46503 | Sisco Laboratories | 1 ml | EUR 23.09 |
Description: Part E |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
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46503-1 | Sisco Laboratories | 5 x1 ml | EUR 70.13 |
Description: Part E |
Roseate PCR Master Mix (2x) (Taq Mix (2x)) |
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46503-2 | Sisco Laboratories | 5 x2.5 ml | EUR 145.39 |
Description: Part E |
Apex Taq RED Master Mix, 2X |
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42-137 | Genesee Scientific | 100 Reactions/Unit | EUR 58.5 |
Description: 1.5mM MgCl2 (Final Conc.) |
Apex Taq RED Master Mix, 2X |
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42-138 | Genesee Scientific | 500 Reactions/Unit | EUR 221.56 |
Description: 1.5mM MgCl2 (Final Conc.) |
Apex Taq RED Master Mix, 2X |
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42-138B | Genesee Scientific | 1000 Reactions/Unit | EUR 437.28 |
Description: 1.5mM MgCl2 (Final Conc.) |
Color Taq PCR Master Mix (2x) |
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E2525-01 | EURx | 100reactions | EUR 38.15 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized and colored reaction buffer, MgCl2 and dNTPs |
Color Taq PCR Master Mix (2x) |
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E2525-02 | EURx | 200reactions | EUR 71.94 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized and colored reaction buffer, MgCl2 and dNTPs |
Color Taq PCR Master Mix (2x) |
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E2525-03 | EURx | 500reactions | EUR 155.87 |
Description: Ready-to-use Solution containing Taq DNA Polymerase, optimized and colored reaction buffer, MgCl2 and dNTPs |
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with ROX, 150rxns |
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QLMM14-R | Vivantis | each | Ask for price |
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with ROX, 150rxns |
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QLMM18-R | Vivantis | each | Ask for price |
ViPrimePLUS Taq qPCR Master Mix, 150rxns |
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QLMM11 | Vivantis | each | Ask for price |
2X hyperfusion plus master mix (With dye) |
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K1117-1 | ApexBio | 1ml | EUR 48 |
Description: Molecular Biology|Reverse Transcription & PCR |
2X hyperfusion plus master mix (With dye) |
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K1117-10 | ApexBio | 10ml | EUR 184 |
Description: Molecular Biology|Reverse Transcription & PCR |
2X hyperfusion plus master mix (With dye) |
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K1117-5 | ApexBio | 5ml | EUR 120 |
Description: Molecular Biology|Reverse Transcription & PCR |
HotStart Taq 2X PCR Master Mix |
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RK20603 | Abclonal | 500RXN | EUR 20.17 |
HotStart Taq (exo-) 2X Master Mix |
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RK20613 | Abclonal | 100RXN | EUR 100.83 |
Perpetual Taq PCR Master Mix (2x) |
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E2740-01 | EURx | 100reactions | EUR 90.47 |
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies,optimized reaction buffer, MgCl2 and dNTPs |
Perpetual Taq PCR Master Mix (2x) |
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E2740-02 | EURx | 200reactions | EUR 174.4 |
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies,optimized reaction buffer, MgCl2 and dNTPs |
Perpetual Taq PCR Master Mix (2x) |
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E2740-03 | EURx | 500reactions | EUR 347.71 |
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies,optimized reaction buffer, MgCl2 and dNTPs |
LongerAmp Taq 2X PCR Master Mix |
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RK20682 | Abclonal | 100RXN | EUR 198.93 |
Gloria Nova HS 2X Master Mix with Dye |
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RK20728 | Abclonal | 1mL | EUR 107.5 |
Pfu-fast 2X PCR Master Mix with Dye |
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RK20654 | Abclonal | 100RXN | EUR 122.63 |
Accuris Taq Master Mix Red Dye, 2X conc., 1000 reactions |
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PR1001-R-1000 | Benchmark Scientific | 1 PC | EUR 375.85 |
Accuris Taq Master Mix Red Dye, 2X conc., 200 reactions |
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PR1001-R-200 | Benchmark Scientific | 1 PC | EUR 90.95 |
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with Low ROX, 150rxns |
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QLMM14-LR | Vivantis | each | Ask for price |
ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with Low ROX, 150rxns |
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QLMM18-LR | Vivantis | each | Ask for price |
FirstTaq 2X PCR Master Mix with GC Buffer with Dye |
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RK20627 | Abclonal | 100RXN | EUR 59.95 |
FirstTaq 2X PCR Master Mix with Standard Buffer with Dye |
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RK20626 | Abclonal | 500RXN | EUR 13.63 |
Color Perpetual Taq PCR Master Mix (2x) |
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E2745-01 | EURx | 100reactions | EUR 90.47 |
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies, optimizedand colored reaction buffer, MgCl2 and dNTPs |
Color Perpetual Taq PCR Master Mix (2x) |
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E2745-02 | EURx | 200reactions | EUR 174.4 |
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies, optimizedand colored reaction buffer, MgCl2 and dNTPs |
Color Perpetual Taq PCR Master Mix (2x) |
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E2745-03 | EURx | 500reactions | EUR 347.71 |
Description: Ready-to-use Solution containing Perpetual Taq DNA Polymerase, antibodies, optimizedand colored reaction buffer, MgCl2 and dNTPs |
Taq Pro Universal SYBR qPCR Master Mix |
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Q712-02 | Vazyme | 500 rxns (20 μl/rxn) | EUR 161 |
Taq Pro Universal SYBR qPCR Master Mix |
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Q712-03 | Vazyme | 2,500 rxns (20 μl/rxn) | EUR 725 |
Taq Pro Universal SYBR qPCR Master Mix |
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Q712-00-100rxns | Vazyme | 100 rxns | EUR 1.09 |
Taq Pro Universal SYBR qPCR Master Mix |
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Q712-02-500rxns | Vazyme | 500 rxns | EUR 166.72 |
Taq Pro Universal SYBR qPCR Master Mix |
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Q712-03-2500rxns | Vazyme | 2,500 rxns | EUR 750.74 |
Accuris Taq Master Mix Red Dye, 2X conc., sample, 5 reactions |
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PR1001-R-S | Benchmark Scientific | 1 PC | EUR 7.31 |
Accuris Taq Master Mix, 2X conc., 1000 reactions |
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PR1001-1000 | Benchmark Scientific | 1 PC | EUR 375.85 |
Accuris Taq Master Mix, 2X conc., 200 reactions |
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PR1001-200 | Benchmark Scientific | 1 PC | EUR 89.71 |
Hi-SYBr Master Mix (with ROX) |
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MBT160-0.5ML | EWC Diagnostics | 1 unit | EUR 52.29 |
Description: Hi-SYBr Master Mix (with ROX) |
Hi-SYBr Master Mix (with ROX) |
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MBT160-1ML | EWC Diagnostics | 1 unit | EUR 94.08 |
Description: Hi-SYBr Master Mix (with ROX) |
Hi-SYBr Master Mix (with ROX) |
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MBT160-5ML | EWC Diagnostics | 1 unit | EUR 376.4 |
Description: Hi-SYBr Master Mix (with ROX) |
2X HyPerFUsion® High-Fidelity Master Mix (With dye) |
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K1030-1 | ApexBio | 1ml | EUR 20 |
2X HyPerFUsion® High-Fidelity Master Mix (With dye) |
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K1030-10 | ApexBio | 10ml | EUR 112 |
2X HyPerFUsion® High-Fidelity Master Mix (With dye) |
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K1030-5 | ApexBio | 5ml | EUR 67 |
Apex Hot Start Taq BLUE Master Mix, 2X |
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42-143 | Genesee Scientific | 100 Reactions/Unit | EUR 65.77 |
Description: 1.5mM MgCl2 (Final) |
Apex Hot Start Taq BLUE Master Mix, 2X |
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42-144 | Genesee Scientific | 500 Reactions/Unit | EUR 288.71 |
Description: 1.5mM MgCl2 (Final) |
Apex Hot Start Taq BLUE Master Mix, 2X |
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42-145 | Genesee Scientific | 1000 Reactions/Unit | EUR 470.2 |
Description: 1.5mM MgCl2 (Final) |
Apex Hot Start Taq BLUE Master Mix, 2X |
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42-146 | Genesee Scientific | 2500 Reactions/Unit | EUR 1216.89 |
Description: 1.5mM MgCl2 (Final), Buffer I |
Apex Hot Start Taq BLUE Master Mix, 2X |
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42-147 | Genesee Scientific | 100 Reactions/Unit | EUR 61.77 |
Description: 1.5mM MgCl2 (Final), Buffer II |
Apex Hot Start Taq BLUE Master Mix, 2X |
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42-148 | Genesee Scientific | 500 Reactions/Unit | EUR 253.97 |
Description: 1.5mM MgCl2 (Final), Buffer II |
Apex Hot Start Taq BLUE Master Mix, 2X |
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42-149 | Genesee Scientific | 1000 Reactions/Unit | EUR 473.03 |
Description: 1.5mM MgCl2 (Final), Buffer II |
Apex Hot Start Taq BLUE Master Mix, 2X |
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42-150 | Genesee Scientific | 2500 Reactions/Unit | EUR 1072.65 |
Description: 1.5mM MgCl2 (Final), Buffer II |
ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns |
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QLMM12 | Vivantis | each | Ask for price |
ViPrimePLUS Taq qPCR Green Master Mix II, 150rxns |
|||
QLMM17 | Vivantis | each | Ask for price |
Accuris Hot Start Taq Master Mix Red Dye, 2X conc., 1000 reactions |
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PR1001-HSR-1000 | Benchmark Scientific | 1 PC | EUR 678.87 |