Przydatność kliniczna cyfrowej PCR kropli
Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów BCR-ABL1 pacjentów z ostrą białaczką limfoblastyczną z dodatnim chromosomem Philadelphia Publish-chimeryczny receptor antygenowy 19/22 T-Cell Cocktail Remedy
Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) accounts for 20-30% of grownup sufferers with ALL, characterised by translocation of t (9, 22). Tyrosine kinase inhibitors (TKIs) have considerably improved the end result although there are nonetheless some issues together with relapse because of drug-resistant mutations and suboptimal molecular remission depth. Beforehand, we reported the protection and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy within the therapy of relapsed/refractory (R/R) B-cell neoplasms together with circumstances with Ph+ ALL. Given potential deeper response, extra sufferers have been anticipated to succeed in optimum minimal residual illness (MRD) response.
Another methodology, duplex droplet digital PCR (ddPCR) with excessive sensitivity was established, which might present absolute quantification of MRD with out the necessity for calibration curves. Right here, we retrospectively collected 95 bone marrow samples from 10 sufferers with R/R Ph+, who obtained 19/22 CAR-T-cell cocktail remedy. Notably, sequential molecular remission for greater than three months (SMR3), a major indicator based mostly on ddPCR after CAR-T infusion was established, which was outlined as a sequential molecular remission for not <three months with damaging MRD. On this cohort, no recurrence was noticed in six sufferers attaining SMR3, the place 4 of whom accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR-T cell routine.
Sadly, the opposite 4 sufferers who didn’t attain SMR3 relapsed, and didn’t obtain further particular therapy besides CAR-T routine. To sum up, ddPCR could also be an alternate, particularly when nucleic acid was inadequate in medical apply. No achievement of SMR3 could be an early warning of potential relapse after CAR-T and indicating the initiation of different therapies together with allo-HSCT.
anti-GFP VHH antibody (Alexa Fluor®647) |
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| JOT0001-5-5 | Jotbody | 5 | EUR 121.8 |
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Description: Conjugated VHH single domain antibody |
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anti-GFP VHH antibody (Alexa Fluor®647) |
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| JOT0001-5-50 | Jotbody | 50 | EUR 340.96 |
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Description: Conjugated VHH single domain antibody |
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anti-GFP VHH antibody (Alexa Fluor®647) |
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| SB-JOT0001-5-100 | Jotbody | 100 (25 x 4) | EUR 340.9 |
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Description: Conjugated VHH single domain antibody |
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anti-GFP VHH antibody (Alexa Fluor®647) |
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| SB-JOT0001-5-25 | Jotbody | 25 | EUR 170.45 |
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Description: Conjugated VHH single domain antibody |
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LAMP3 (human) monoclonal antibody (104G4) (Alexa Fluor 647) |
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| MBS566787-01mg | MyBiosource | 0.1mg | EUR 620 |
LAMP3 (human) monoclonal antibody (104G4) (Alexa Fluor 647) |
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| MBS566787-5x01mg | MyBiosource | 5x0.1mg | EUR 2740 |
CD39 (human) monoclonal antibody (AC2.5) (Alexa Fluor 647) |
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| MBS566808-01mg | MyBiosource | 0.1mg | EUR 650 |
CD39 (human) monoclonal antibody (AC2.5) (Alexa Fluor 647) |
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| MBS566808-5x01mg | MyBiosource | 5x0.1mg | EUR 2865 |
anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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| JOT0002-5-1 | Jotbody | each | Ask for price |
anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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| JOT0002-5-4-15 | Jotbody | 15 | EUR 221.9 |
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Description: Conjugated VHH single domain antibody |
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anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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| JOT0002-5-4-5 | Jotbody | 5 | EUR 121.8 |
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Description: Conjugated VHH single domain antibody |
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anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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| JOT0002-5-4-50 | Jotbody | 50 | EUR 340.96 |
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Description: Conjugated VHH single domain antibody |
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anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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| SB-JOT0002-5-4-100 | Jotbody | 100 (25 x 4) | EUR 340.9 |
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Description: Conjugated VHH single domain antibody |
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anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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| SB-JOT0002-5-4-25 | Jotbody | 25 | EUR 170.45 |
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Description: Conjugated VHH single domain antibody |
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Alexa Fluor® 647 anti-human IL-1β Antibody |
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| E16FHKIL1-100 | EnoGene | 100Tests | EUR 945 |
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Description: Available in various conjugation types. |
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SAM FCM (Alexa Fluor 647) |
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| abx098902-100tests | Abbexa | 100 tests | EUR 1479.6 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459433-25Tests | MyBiosource | 25Tests | EUR 230 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459433-50Tests | MyBiosource | 50Tests | EUR 290 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459433-5x50Tests | MyBiosource | 5x50Tests | EUR 1160 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459434-25Tests | MyBiosource | 25Tests | EUR 230 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459434-50Tests | MyBiosource | 50Tests | EUR 290 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459434-5x50Tests | MyBiosource | 5x50Tests | EUR 1160 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459435-25Tests | MyBiosource | 25Tests | EUR 230 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459435-50Tests | MyBiosource | 50Tests | EUR 290 |
Alexa Fluor 647 anti-human CD3 |
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| MBS9459435-5x50Tests | MyBiosource | 5x50Tests | EUR 1160 |
Alexa Fluor® 647 Streptavidin |
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| E16FXP040K | EnoGene | 100 μg | EUR 758.33 |
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Description: Available in various conjugation types. |
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Anti-Mouse NK1.1 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556807-100Tests | MyBiosource | 100Tests | EUR 130 |
Anti-Mouse NK1.1 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556807-200Tests | MyBiosource | 200Tests | EUR 155 |
Anti-Mouse NK1.1 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556807-50Tests | MyBiosource | 50Tests | EUR 110 |
Anti-Mouse NK1.1 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556807-5x200Tests | MyBiosource | 5x200Tests | EUR 685 |
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556821-100Tests | MyBiosource | 100Tests | EUR 165 |
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556821-200Tests | MyBiosource | 200Tests | EUR 215 |
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556821-20Tests | MyBiosource | 20Tests | EUR 115 |
Anti-Human CD3 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556821-5x200Tests | MyBiosource | 5x200Tests | EUR 950 |
Anti-Human CD5 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556856-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD5 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556856-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD5 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556856-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD5 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556856-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Mouse CD4 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556901-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD4 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556901-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD4 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556901-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD4 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556901-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Human CD4 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556914-100Tests | MyBiosource | 100Tests | EUR 165 |
Anti-Human CD4 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556914-200Tests | MyBiosource | 200Tests | EUR 215 |
Anti-Human CD4 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556914-20Tests | MyBiosource | 20Tests | EUR 130 |
Anti-Human CD4 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556914-5x200Tests | MyBiosource | 5x200Tests | EUR 950 |
Anti-Human CD8 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556916-100Tests | MyBiosource | 100Tests | EUR 165 |
Anti-Human CD8 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556916-200Tests | MyBiosource | 200Tests | EUR 215 |
Anti-Human CD8 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556916-20Tests | MyBiosource | 20Tests | EUR 115 |
Anti-Human CD8 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556916-5x200Tests | MyBiosource | 5x200Tests | EUR 950 |
Anti-Mouse CD3 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556411-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD3 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556411-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD3 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556411-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD3 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556411-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Mouse CD19 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556805-100Tests | MyBiosource | 100Tests | EUR 130 |
Anti-Mouse CD19 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556805-200Tests | MyBiosource | 200Tests | EUR 155 |
Anti-Mouse CD19 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556805-50Tests | MyBiosource | 50Tests | EUR 110 |
Anti-Mouse CD19 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556805-5x200Tests | MyBiosource | 5x200Tests | EUR 685 |
Anti-Human CD86 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556829-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Human CD86 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556829-200Tests | MyBiosource | 200Tests | EUR 265 |
Anti-Human CD86 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556829-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD86 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556829-5x200Tests | MyBiosource | 5x200Tests | EUR 1175 |
Anti-Mouse CD28 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556840-100Tests | MyBiosource | 100Tests | EUR 155 |
Anti-Mouse CD28 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556840-200Tests | MyBiosource | 200Tests | EUR 190 |
Anti-Mouse CD28 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556840-50Tests | MyBiosource | 50Tests | EUR 125 |
Anti-Mouse CD28 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556840-5x200Tests | MyBiosource | 5x200Tests | EUR 855 |
Anti-Mouse CD40 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556843-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD40 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556843-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD40 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556843-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD40 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556843-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Human CD29 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556861-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD29 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556861-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD29 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556861-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD29 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556861-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Human CD18 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556869-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD18 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556869-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD18 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556869-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD18 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556869-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Human CD38 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556871-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD38 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556871-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD38 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556871-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD38 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556871-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Human CD47 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556873-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Human CD47 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556873-200Tests | MyBiosource | 200Tests | EUR 265 |
Anti-Human CD47 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556873-20Tests | MyBiosource | 20Tests | EUR 140 |
Anti-Human CD47 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556873-5x200Tests | MyBiosource | 5x200Tests | EUR 1175 |
Anti-Mouse CD25 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556905-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD25 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556905-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD25 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556905-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD25 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556905-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Mouse CD8a Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556908-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD8a Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556908-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD8a Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556908-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD8a Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556908-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Mouse Ly6G Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556912-100Tests | MyBiosource | 100Tests | EUR 135 |
Anti-Mouse Ly6G Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556912-200Tests | MyBiosource | 200Tests | EUR 180 |
Anti-Mouse Ly6G Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556912-50Tests | MyBiosource | 50Tests | EUR 115 |
Anti-Mouse Ly6G Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556912-5x200Tests | MyBiosource | 5x200Tests | EUR 795 |
Anti-Mouse CD86 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2557987-100Tests | MyBiosource | 100Tests | EUR 185 |
Anti-Mouse CD86 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2557987-200Tests | MyBiosource | 200Tests | EUR 235 |
Anti-Mouse CD86 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2557987-50Tests | MyBiosource | 50Tests | EUR 140 |
Anti-Mouse CD86 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2557987-5x200Tests | MyBiosource | 5x200Tests | EUR 1045 |
Anti-Human CD2 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2559103-100Tests | MyBiosource | 100Tests | EUR 235 |
Anti-Human CD2 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2559103-200Tests | MyBiosource | 200Tests | EUR 300 |
Anti-Human CD2 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2559103-20Tests | MyBiosource | 20Tests | EUR 140 |
Anti-Human CD2 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2559103-5x200Tests | MyBiosource | 5x200Tests | EUR 1350 |
Anti-Human CD20 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558336-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD20 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558336-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD20 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558336-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD20 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558336-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Human CD32 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558467-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Human CD32 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558467-200Tests | MyBiosource | 200Tests | EUR 265 |
Anti-Human CD32 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558467-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD32 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558467-5x200Tests | MyBiosource | 5x200Tests | EUR 1175 |
Anti-Human CD64 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558504-100Tests | MyBiosource | 100Tests | EUR 225 |
Anti-Human CD64 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558504-200Tests | MyBiosource | 200Tests | EUR 290 |
Anti-Human CD64 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558504-20Tests | MyBiosource | 20Tests | EUR 135 |
Anti-Human CD64 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558504-5x200Tests | MyBiosource | 5x200Tests | EUR 1305 |
Anti-Human CD41 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558518-100Tests | MyBiosource | 100Tests | EUR 220 |
Anti-Human CD41 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558518-200Tests | MyBiosource | 200Tests | EUR 315 |
Anti-Human CD41 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558518-20Tests | MyBiosource | 20Tests | EUR 150 |
Anti-Human CD41 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558518-5x200Tests | MyBiosource | 5x200Tests | EUR 1410 |
Anti-Mouse CD71 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558574-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD71 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558574-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD71 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558574-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD71 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558574-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Mouse CD90.2 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558589-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD90.2 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558589-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD90.2 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558589-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD90.2 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2558589-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Mouse CD48 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556416-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD48 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556416-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD48 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556416-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD48 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556416-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Mouse CD54 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556421-100Tests | MyBiosource | 100Tests | EUR 115 |
Anti-Mouse CD54 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556421-200Tests | MyBiosource | 200Tests | EUR 135 |
Anti-Mouse CD54 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556421-50Tests | MyBiosource | 50Tests | EUR 100 |
Anti-Mouse CD54 Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556421-5x200Tests | MyBiosource | 5x200Tests | EUR 610 |
Anti-Mouse CD1d Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556434-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD1d Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556434-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD1d Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556434-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD1d Monoclonal Antibody (Alexa Fluor 647 Conjugated) |
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| MBS2556434-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Mouse CD11c Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556811-100Tests | MyBiosource | 100Tests | EUR 130 |
Anti-Mouse CD11c Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556811-200Tests | MyBiosource | 200Tests | EUR 155 |
Anti-Mouse CD11c Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556811-50Tests | MyBiosource | 50Tests | EUR 110 |
Anti-Mouse CD11c Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556811-5x200Tests | MyBiosource | 5x200Tests | EUR 685 |
Anti-Mouse CD127 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556837-100Tests | MyBiosource | 100Tests | EUR 200 |
Anti-Mouse CD127 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556837-200Tests | MyBiosource | 200Tests | EUR 255 |
Anti-Mouse CD127 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556837-50Tests | MyBiosource | 50Tests | EUR 155 |
Anti-Mouse CD127 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556837-5x200Tests | MyBiosource | 5x200Tests | EUR 1150 |
Anti-Mouse CD122 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556846-100Tests | MyBiosource | 100Tests | EUR 135 |
Anti-Mouse CD122 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556846-200Tests | MyBiosource | 200Tests | EUR 180 |
Anti-Mouse CD122 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556846-50Tests | MyBiosource | 50Tests | EUR 115 |
Anti-Mouse CD122 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556846-5x200Tests | MyBiosource | 5x200Tests | EUR 795 |
Anti-Human CD49d Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556854-100Tests | MyBiosource | 100Tests | EUR 235 |
Anti-Human CD49d Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556854-200Tests | MyBiosource | 200Tests | EUR 310 |
Anti-Human CD49d Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556854-20Tests | MyBiosource | 20Tests | EUR 150 |
Anti-Human CD49d Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556854-5x200Tests | MyBiosource | 5x200Tests | EUR 1395 |
Anti-Mouse CD103 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556895-100Tests | MyBiosource | 100Tests | EUR 265 |
Anti-Mouse CD103 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556895-200Tests | MyBiosource | 200Tests | EUR 360 |
Anti-Mouse CD103 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556895-50Tests | MyBiosource | 50Tests | EUR 195 |
Anti-Mouse CD103 Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556895-5x200Tests | MyBiosource | 5x200Tests | EUR 1610 |
Anti-Mouse CD49b Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556924-100Tests | MyBiosource | 100Tests | EUR 140 |
Anti-Mouse CD49b Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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| MBS2556924-200Tests | MyBiosource | 200Tests | EUR 185 |
Anti-Mouse CD49b Monoclonal Antibody (Alexa Fluor 647 Conjuaged) |
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Ilościowe wykrywanie i monitorowanie Colletotrichum siamense na drzewach kauczukowych przy użyciu PCR w czasie rzeczywistym
Colletotrichum siamense is among the most necessary pathogens of rubber bushes in Asia. The correct detection and quantification of C. siamense populations in rubber bushes are of significance for monitoring the epidemics of the illness. On this research, we developed an ITS-based real-time PCR methodology to effectively detect C. siamense infecting rubber tree, which reliably detected as little as 100 fg genomic DNA, 100 copies of goal DNA and 20 conidia.
The true-time PCR protocol acknowledged all C. siamense isolates collected from three provinces in China, whereas no amplification was noticed with rubber tree and its different pathogens. Detection and quantification of C. siamense have been carried out in artificially and naturally contaminated rubber leaves. We might nonetheless detect C. siamense in plant mixes of which solely 0.0001% of the tissue contaminated.
An accumulation of C. siamense DNA was noticed throughout the entire an infection course of in any respect three leaf phenological levels, suggesting the real-time PCR methodology can be utilized to watch C. siamense growth in rubber bushes. Lastly, the tactic allowed the detection of C. siamense in naturally contaminated and symptomless leaves of rubber bushes within the fields. In contrast with earlier detection strategies, the real-time PCR methodology is extra particular and extra delicate, and can be of nice use for research aiming to achieve a greater understanding of the epidemiology of Colletotrichum leaf illness, in addition to the prediction of illness threat and the management proposal.
Ilościowy pomiar liczby kopii transpozonu przy użyciu cyfrowej PCR kropli
Spontaneous proliferation of transposable parts contributes to genetic variety at various ranges equivalent to somatic mosaicism, genetic divergence in inhabitants, and genome evolution. Such genetic variety is crucial for vegetation’ adaptation to altering atmosphere and serves as a beneficial useful resource for crop enchancment. Due to this fact, measuring the copy quantity variation of transposable parts with precision and effectivity is necessary to grasp the extent of their proliferation.
Droplet Digital PCR (ddPCR) is an correct and delicate method that enables measurement of copy quantity variation of a transposon. Briefly, genomic DNA is extracted, digested, and partitioned into hundreds of nanoliter-scale droplets. The TaqMan real-time PCR adopted by the end-point fluorescence detection allows the quantitative measurement of copy variety of template DNAs. Right here on this chapter, we describe the step-by-step process of ddPCR utilizing EVADE retrotransposon of Arabidopsis for example.
Porównanie wyników tomografii komputerowej (CT) z RT-PCR w diagnostyce COVID-19 u dzieci
polymerase chain response (RT-PCR) check ends in youngsters with possible or definitive analysis of coronavirus illness 2019 (COVID-19).
Strategies: On this retrospective archive research, pediatric sufferers who have been adopted up within the hospital with a potential or definitive analysis of COVID-19 and who had chest CT at presentation have been included. CT scan photos of the sufferers have been reinterpreted by a pediatric radiologist and in contrast with their RT-PCR check outcomes.
Outcomes: Of the entire of 89 sufferers, 33 had damaging and 56 had optimistic RT-PCR exams. The presence of pulmonary lesions and consolidation was statistically considerably greater within the RT-PCR damaging group than within the RT-PCR optimistic group (p = 0.037 and 0.001, respectively). Lobe involvement of 0%-25% was greater within the RT-PCR optimistic group (p = 0.001), and lobe involvements of 25%-50% and 50%-75% have been considerably greater within the RT-PCR damaging group (p = 0.001 and 0.005, respectively). Central and perihilar involvement was discovered to be statistically important in the RT-PCR damaging group (p = 0.008 and 0.005, respectively).
Conclusion: Chest CT findings could present some clues in predicting RT-PCR positivity in youngsters with a possible analysis of COVID-19. Lobe involvement share of as much as 25% is a discovering in favor of sufferers with optimistic RT-PCR check, whereas 25%-75% lobe involvement, central and perihilar involvement, and consolidation may be interpreted in favor of sufferers with damaging RT-PCR check.
Integracja przygotowania próbek mikroprzepływowych z detekcją PCR w celu zbadania skutków jednoczesnej separacji inhibitora DNA i wymiany roztworu DNA
- On this paper, we utilized a curved-channel microfluidic machine to separate DNA from PCR-inhibitor-containing water and concurrently wash them into clear water for detection utilizing a transportable PCR thermocycler. Environmental DNA (eDNA) sampling has develop into an efficient surveying method for detecting uncommon organisms. Nevertheless, low focus eDNA molecules could also be masked by PCR inhibitors throughout amplification and detection, growing the danger of false negatives. Due to this fact, applied sciences for on-site DNA separation and washing are urgently wanted.
- Our machine consisted of a half-circle microchannel with a DNA-inhibitor pattern inlet, a clear buffer inlet, and a number of shops. By utilizing the flow-induced inertial forces, 10 μm DNA-conjugated microparticles have been targeted on the inner-wall of the curved microchannel whereas separation from 1 μm inhibitor-conjugated microparticles and DNA washing have been achieved concurrently with the Dean stream.
- We achieved singleplex focusing, isolation and washing of 10 μm particles at an effectivity of 94.5 ± 2.0%. In duplex experiments with 1 μm and 10 μm particles, bigger particles have been washed with an effectivity of 92.1 ± 1.6% and a purity of 79 ± 2%. By surface-functionalizing the microparticles with affinity teams in opposition to Atlantic salmon DNA and humic acid (HA), and processing samples of assorted concentrations in our machine, we achieved an efficient purification and detection of DNA molecules utilizing the moveable PCR thermocycler.
- Our methodology considerably decreased PCR quantitation cycles from Cq > 38 to Cq = 30.35 ± 0.5, which confirmed enhancement of PCR amplification. The proposed machine takes a promising step ahead in pattern preparation in the direction of an built-in machine that can be utilized for simultaneous purification and resolution alternate of DNA in point-of-need environmental monitoring functions.
