• 5 maja, 2021
ewaagroart

Przydatność kliniczna cyfrowej PCR kropli

Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów BCR-ABL1 pacjentów z ostrą białaczką limfoblastyczną z dodatnim chromosomem Philadelphia Publish-chimeryczny receptor antygenowy 19/22 T-Cell Cocktail Remedy 

Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) accounts for 20-30% of grownup sufferers with ALL, characterised by translocation of t (9, 22). Tyrosine kinase inhibitors (TKIs) have considerably improved the end result although there are nonetheless some issues together with relapse because of drug-resistant mutations and suboptimal molecular remission depth. Beforehand, we reported the protection and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy within the therapy of relapsed/refractory (R/R) B-cell neoplasms together with circumstances with Ph+ ALL. Given potential deeper response, extra sufferers have been anticipated to succeed in optimum minimal residual illness (MRD) response.

Another methodology, duplex droplet digital PCR (ddPCR) with excessive sensitivity was established, which might present absolute quantification of MRD with out the necessity for calibration curves. Right here, we retrospectively collected 95 bone marrow samples from 10 sufferers with R/R Ph+, who obtained 19/22 CAR-T-cell cocktail remedy. Notably, sequential molecular remission for greater than three months (SMR3), a major indicator based mostly on ddPCR after CAR-T infusion was established, which was outlined as a sequential molecular remission for not <three months with damaging MRD. On this cohort, no recurrence was noticed in six sufferers attaining SMR3, the place 4 of whom accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR-T cell routine.

Sadly, the opposite 4 sufferers who didn’t attain SMR3 relapsed, and didn’t obtain further particular therapy besides CAR-T routine. To sum up, ddPCR could also be an alternate, particularly when nucleic acid was inadequate in medical apply. No achievement of SMR3 could be an early warning of potential relapse after CAR-T and indicating the initiation of different therapies together with allo-HSCT.

 

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anti-GFP VHH antibody (Alexa Fluor® 647)

JOT0001-3-5 5
EUR 121.77
Description: Conjugated VHH single domain antibody

anti-GFP VHH antibody (Alexa Fluor® 647)

JOT0001-3-50 50
EUR 340.96
Description: Conjugated VHH single domain antibody

anti-PD-L1 VHH antibody (Alexa Fluor® 647)

JOT0002-5-1 each Ask for price

anti-PD-L1 VHH antibody (Alexa Fluor® 647)

JOT0002-5-2-15 15
EUR 221.92
Description: Primary VHH single domain antibody

anti-PD-L1 VHH antibody (Alexa Fluor® 647)

JOT0002-5-2-5 5
EUR 121.77
Description: Primary VHH single domain antibody

anti-PD-L1 VHH antibody (Alexa Fluor® 647)

JOT0002-5-2-50 50
EUR 340.96
Description: Primary VHH single domain antibody

Alexa Fluor® 647 anti-human IL-1β Antibody

E16FHKIL1-100 100Tests
EUR 945
Description: Available in various conjugation types.

SAM FCM (Alexa Fluor 647)

abx098902-100tests 100 tests
EUR 1479.6

Alexa Fluor® 647 Streptavidin

E16FXP040K 100 μg
EUR 758.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-rat CD3

E16FRK003-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-rat CD8a

E16FRK008a-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-rat CD43

E16FRK043-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-rat CD45

E16FRK045-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-rat CD45

E16FRK045-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-rat CD86

E16FRK086-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 rat IgG2a

E16FRCK002-100U 0.1 mg
EUR 628.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse IgM

E16SMK501-025U 25 μg
EUR 325
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse IgM

E16SMK501-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD3

E16FMK003-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD4

E16FMK004-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD5

E16FMK005-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD5

E16FMK005-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD9

E16FMK009-025U 25 μg
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD9

E16FMK009-100U 100 μg
EUR 931.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse IgD

E16SDK501-025U 25 μg
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse IgD

E16SDK501-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD3

E16FHK003-025 25 tests
EUR 288.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD3

E16FHK003-050 50 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD3

E16FHK003-100 100 tests
EUR 682.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD4

E16FHK004-025 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD4

E16FHK004-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD5

E16FHK005-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD8

E16FHK008-025 25 tests
EUR 288.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD8

E16FHK008-050 50 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD8

E16FHK008-100 100 tests
EUR 682.67
Description: Available in various conjugation types.

Alexa Fluor® 647 mouse IgG1

E16FMCK001-100 100 tests
EUR 736.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse IgG1

E16SHK501-025U 25 μg
EUR 325
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse IgG1

E16SHK501-100U 100 μg
EUR 715
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD3e

E16FMK003e-025U 25 μg
EUR 362.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD8a

E16FMK008a-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD18

E16FMK018-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD19

E16FMK019-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD22

E16FMK022-025U 25 μg
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD22

E16FMK022-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD23

E16FMK023-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD23

E16FMK023-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD24

E16FMK024-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD24

E16FMK024-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD25

E16FMK025-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD25

E16FMK025-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD31

E16FMK031-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD34

E16FMK034-025U 25 μg
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD34

E16FMK034-100U 100 μg
EUR 888.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD36

E16FMK036-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD39

E16FMK039-025U 25 μg
EUR 498.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD39

E16FMK039-100U 100 μg
EUR 1278.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD40

E16FMK040-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD40

E16FMK040-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD45

E16FMK045-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD45.2

E16FMK045.2-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD47

E16FMK047-025U 25 μg
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD47

E16FMK047-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD48

E16FMK048-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD54

E16FMK054-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD68

E16FMK068-025U 25 μg
EUR 541.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD68

E16FMK068-100U 100 μg
EUR 1278.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD69

E16FMK069-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD73

E16FMK073-025U 25 μg
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD73

E16FMK073-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD80

E16FMK080-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD80

E16FMK080-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD83

E16FMK083-100U 100 μg
EUR 1191.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD86

E16FMK086-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD86

E16FMK086-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD90.2

E16FMK090.2-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-rat CD45RC

E16FRK045RC-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD1a

E16FHK001a-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD1c

E16FHK001c-100 100 tests
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD8a

E16FHK008a-025 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD8a

E16FHK008a-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD14

E16FHK0142-025 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD14

E16FHK0142-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD16

E16FHK016-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD16

E16FHK016-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD19

E16FHK019-025 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD19

E16FHK019-100 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD20

E16FHK020-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD24

E16FHK024-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD24

E16FHK024-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD25

E16FHK025-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD25

E16FHK025-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD27

E16FHK027-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD29

E16FHK029-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD29

E16FHK029-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD31

E16FHK031-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD31

E16FHK031-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD32

E16FHK032-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD34

E16FHK034-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD34

E16FHK034-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD38

E16FHK038-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD40

E16FHK040-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD40

E16FHK040-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD45

E16FHK045-025 25 tests
EUR 288.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD45

E16FHK045-050 50 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD45

E16FHK045-100 100 tests
EUR 682.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD54

E16FHK054-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD57

E16FHK057-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD61

E16FHK061-025 25 tests
EUR 520
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD61

E16FHK061-100 100 tests
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD63

E16FHK063-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD63

E16FHK063-100 100 tests
EUR 1170
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD64

E16FHK064-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD69

E16FHK069-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD80

E16FHK080-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD80

E16FHK080-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD82

E16FHK082-100 100 tests
EUR 1148.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD83

E16FHK083-100 100 tests
EUR 1083.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD86

E16FHK086-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD86

E16FHK086-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD94

E16FHK094-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse IgG2a

E16SKK501-025U 25 μg
EUR 325
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse IgG2a

E16SKK501-100U 100 μg
EUR 715
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD11a

E16FMK011a-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD11a

E16FMK011a-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD11c

E16FMK011c-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD11c

E16FMK011c-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD49b

E16FMK049b-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD49d

E16FMK049d-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD49d

E16FMK049d-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD49e

E16FMK049e-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD62L

E16FMK062L-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD102

E16FMK102-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD103

E16FMK103-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD104

E16FMK104-025U 25 μg
EUR 585
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD104

E16FMK104-100U 100 μg
EUR 1365
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD106

E16FMK106-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-mouse CD106

E16FMK106-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD11a

E16FHK011a-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD11b

E16FHK011b-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD11b

E16FHK011b-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD11b

E16FHK011b-100U 100 μg
EUR 845
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD11c

E16FHK011c-025 25 tests
EUR 411.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD11c

E16FHK011c-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD11c

E16FHK011c-100U 100 μg
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD49a

E16FHK049a-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD62L

E16FHK062L-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD66b

E16FHK066b-025 25 tests
EUR 541.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD66b

E16FHK066b-100 100 tests
EUR 1191.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD105

E16FHK105-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD123

E16FHK123-025 25 tests
EUR 563.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD123

E16FHK123-100 100 tests
EUR 1148.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD146

E16FHK146-025 25 tests
EUR 476.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD146

E16FHK146-100 100 tests
EUR 1061.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD147

E16FHK147-025 25 tests
EUR 455
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD147

E16FHK147-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD154

E16FHK154-100 100 tests
EUR 975
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD160

E16FHK160-025 25 tests
EUR 650
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD160

E16FHK160-100 100 tests
EUR 1408.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD161

E16FHK161-025 25 tests
EUR 541.67
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD161

E16FHK161-100 100 tests
EUR 1278.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD163

E16FHK163-025 25 tests
EUR 433.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD163

E16FHK163-100 100 tests
EUR 975
Description: Available in various conjugation types.

Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 647

nAP-20508 500ug
EUR 183.6

Alexa Fluor® 647 anti-mouse CD45RB

E16FMK045RB-025U 25 μg
EUR 368.33
Description: Available in various conjugation types.

Alexa Fluor® 647 anti-human CD45RA

E16FHK0453-025 25 tests
EUR 455
Description: Available in various conjugation types.

Ilościowe wykrywanie i monitorowanie Colletotrichum siamense na drzewach kauczukowych przy użyciu PCR w czasie rzeczywistym

Colletotrichum siamense is among the most necessary pathogens of rubber bushes in Asia. The correct detection and quantification of C. siamense populations in rubber bushes are of significance for monitoring the epidemics of the illness. On this research, we developed an ITS-based real-time PCR methodology to effectively detect C. siamense infecting rubber tree, which reliably detected as little as 100 fg genomic DNA, 100 copies of goal DNA and 20 conidia.

The true-time PCR protocol acknowledged all C. siamense isolates collected from three provinces in China, whereas no amplification was noticed with rubber tree and its different pathogens. Detection and quantification of C. siamense have been carried out in artificially and naturally contaminated rubber leaves. We might nonetheless detect C. siamense in plant mixes of which solely 0.0001% of the tissue contaminated.

An accumulation of C. siamense DNA was noticed throughout the entire an infection course of in any respect three leaf phenological levels, suggesting the real-time PCR methodology can be utilized to watch C. siamense growth in rubber bushes. Lastly, the tactic allowed the detection of C. siamense in naturally contaminated and symptomless leaves of rubber bushes within the fields. In contrast with earlier detection strategies, the real-time PCR methodology is extra particular and extra delicate, and can be of nice use for research aiming to achieve a greater understanding of the epidemiology of Colletotrichum leaf illness, in addition to the prediction of illness threat and the management proposal.

Ilościowy pomiar liczby kopii transpozonu przy użyciu cyfrowej PCR kropli 

Spontaneous proliferation of transposable parts contributes to genetic variety at various ranges equivalent to somatic mosaicism, genetic divergence in inhabitants, and genome evolution. Such genetic variety is crucial for vegetation’ adaptation to altering atmosphere and serves as a beneficial useful resource for crop enchancment. Due to this fact, measuring the copy quantity variation of transposable parts with precision and effectivity is necessary to grasp the extent of their proliferation.

Droplet Digital PCR (ddPCR) is an correct and delicate method that enables measurement of copy quantity variation of a transposon. Briefly, genomic DNA is extracted, digested, and partitioned into hundreds of nanoliter-scale droplets. The TaqMan real-time PCR adopted by the end-point fluorescence detection allows the quantitative measurement of copy variety of template DNAs. Right here on this chapter, we describe the step-by-step process of ddPCR utilizing EVADE retrotransposon of Arabidopsis for example.

Porównanie wyników tomografii komputerowej (CT) z RT-PCR w diagnostyce COVID-19 u dzieci

polymerase chain response (RT-PCR) check ends in youngsters with possible or definitive analysis of coronavirus illness 2019 (COVID-19).

Strategies: On this retrospective archive research, pediatric sufferers who have been adopted up within the hospital with a potential or definitive analysis of COVID-19 and who had chest CT at presentation have been included. CT scan photos of the sufferers have been reinterpreted by a pediatric radiologist and in contrast with their RT-PCR check outcomes.

Outcomes: Of the entire of 89 sufferers, 33 had damaging and 56 had optimistic RT-PCR exams. The presence of pulmonary lesions and consolidation was statistically considerably greater within the RT-PCR damaging group than within the RT-PCR optimistic group (p = 0.037 and 0.001, respectively). Lobe involvement of 0%-25% was greater within the RT-PCR optimistic group (p = 0.001), and lobe involvements of 25%-50% and 50%-75% have been considerably greater within the RT-PCR damaging group (p = 0.001 and 0.005, respectively). Central and perihilar involvement was discovered to be statistically important in the RT-PCR damaging group (p = 0.008 and 0.005, respectively).

Conclusion: Chest CT findings could present some clues in predicting RT-PCR positivity in youngsters with a possible analysis of COVID-19. Lobe involvement share of as much as 25% is a discovering in favor of sufferers with optimistic RT-PCR check, whereas 25%-75% lobe involvement, central and perihilar involvement, and consolidation may be interpreted in favor of sufferers with damaging RT-PCR check.

Integracja przygotowania próbek mikroprzepływowych z detekcją PCR w celu zbadania skutków jednoczesnej separacji inhibitora DNA i wymiany roztworu DNA 

  • On this paper, we utilized a curved-channel microfluidic machine to separate DNA from PCR-inhibitor-containing water and concurrently wash them into clear water for detection utilizing a transportable PCR thermocycler. Environmental DNA (eDNA) sampling has develop into an efficient surveying method for detecting uncommon organisms. Nevertheless, low focus eDNA molecules could also be masked by PCR inhibitors throughout amplification and detection, growing the danger of false negatives. Due to this fact, applied sciences for on-site DNA separation and washing are urgently wanted.
  • Our machine consisted of a half-circle microchannel with a DNA-inhibitor pattern inlet, a clear buffer inlet, and a number of shops. By utilizing the flow-induced inertial forces, 10 μm DNA-conjugated microparticles have been targeted on the inner-wall of the curved microchannel whereas separation from 1 μm inhibitor-conjugated microparticles and DNA washing have been achieved concurrently with the Dean stream.
  • We achieved singleplex focusing, isolation and washing of 10 μm particles at an effectivity of 94.5 ± 2.0%. In duplex experiments with 1 μm and 10 μm particles, bigger particles have been washed with an effectivity of 92.1 ± 1.6% and a purity of 79 ± 2%. By surface-functionalizing the microparticles with affinity teams in opposition to Atlantic salmon DNA and humic acid (HA), and processing samples of assorted concentrations in our machine, we achieved an efficient purification and detection of DNA molecules utilizing the moveable PCR thermocycler.
  • Our methodology considerably decreased PCR quantitation cycles from Cq > 38 to Cq = 30.35 ± 0.5, which confirmed enhancement of PCR amplification. The proposed machine takes a promising step ahead in pattern preparation in the direction of an built-in machine that can be utilized for simultaneous purification and resolution alternate of DNA in point-of-need environmental monitoring functions.

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