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Wariant genu SARS-CoV-2 E zmienia

• Ewa
• May 12, 2021April 30, 2021
• 0

Wariant genu SARS-CoV-2 E zmienia charakterystykę czułości analitycznej wykrywania wirusów przy użyciu komercyjnego testu RT-PCR 

Diagnostic assays for detecting SARS-CoV-2 are important for affected person administration, an infection prevention, and the general public well being response for COVID-19. The efficacy and reliability of those assays are of paramount significance in each monitoring and controlling unfold of the virus. Actual-time RT-PCR assays depend on a set genetic sequence for primers and probe binding. Mutations can probably alter the accuracy of those assays and result in unpredictable analytical efficiency traits and false-negative outcomes. Herein, we establish a G-to-U transversion (nucleotide 26372) in the SARS-CoV-2 E gene in three specimens with lowered viral detection effectivity utilizing a extensively accessible industrial assay.

Additional evaluation of the general public GISAID repository led to the identification of 18 further genomes with this mutation, which replicate 5 unbiased mutational occasions. This work helps using dual-target assays to cut back the variety of false-negative PCR outcomes.

hsa-mir-521 Real-time RT-PCR Detection Kit
20-abx097600	Abbexa	EUR 551.00 EUR 787.00 EUR 398.00	100 rxns 200 rxns 50 rxns
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dsGreen for Real-Time PCR, 100×, 100 uL
11010	lumiprobe	100 uL	EUR 59

dsGreen for Real-Time PCR, 100×, 2 mL
61010	lumiprobe	2 mL	EUR 363

dsGreen for Real-Time PCR, 100×, 1.5 mL
51010	lumiprobe	1.5 mL	EUR 331

dsGreen for Real-Time PCR, 100×, 1 mL
41010	lumiprobe	1 mL	EUR 249

dsGreen for Real-Time PCR, 100×, 10 mL
81010	lumiprobe	10 mL	EUR 869

dsGreen for Real-Time PCR, 100×, 5 mL
71010	lumiprobe	5 mL	EUR 625

dsGreen for Real-Time PCR, 100×, 50 mL
91010	lumiprobe	50 mL	EUR 3169

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye
MB1004-100Reactions	BosterBio	100 Reactions	EUR 327

PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)
31033	Biotium	1kit	EUR 428
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PMA Real-Time PCR Bacterial Viability Kit - Mycobacterium tuberculosis (groEL2)
31034	Biotium	1kit	EUR 399
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31035	Biotium	1kit	EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Staphylococcus aureus (mecA)
31036	Biotium	1kit	EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - E. coli (uidA)
31050	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly)
31051	Biotium	1kit	EUR 399
Description: Minimum order quantity: 1 unit of 1kit

PMA Real-Time PCR Bacterial Viability Kit - Legionella pneumophila (mip)
31053	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

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31033-X	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

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31050-X	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

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31051-X	Biotium	1kit	EUR 428
Description: Minimum order quantity: 1 unit of 1kit

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit
RR-0478-02	Liferiver	25 tests/kit	EUR 991
For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl Show more
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

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PCR Mycoplasma Detection Kit
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Human Lung Cancer PCR Primer Library
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31037-X	Biotium	1kit	EUR 428
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0,1ML THIN WALL TUBES 8 PER STRIP. CLEAR & REAL TIME STRIP CAPS
PCR-0108-LP-RT-C	CORNING	125/pk	EUR 709
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hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097625	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
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hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097632	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
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hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097662	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
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hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097663	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
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hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097664	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
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hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
20-abx097665	Abbexa	EUR 732.00 EUR 1038.00 EUR 523.00	100 rxns 200 rxns 50 rxns
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hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit
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Szacowanie skuteczności rutynowych bezobjawowych testów PCR z różnymi częstotliwościami w celu wykrycia zakażeń SARS-CoV-2 

Background: Routine asymptomatic testing utilizing RT-PCR of people that work together with weak populations, comparable to medical workers in hospitals or care staff in care properties, has been employed to assist stop outbreaks amongst weak populations. Though the height sensitivity of RT-PCR could be excessive, the chance of detecting an an infection will range all through the course of an an infection. The effectiveness of routine asymptomatic testing will subsequently depend upon testing frequency and the way PCR detection varies over time.

Strategies: We fitted a Bayesian statistical mannequin to a dataset of twice weekly PCR exams of UK healthcare staff carried out by self-administered nasopharyngeal swab, no matter signs. We collectively estimated instances of an infection and the chance of a optimistic PCR take a look at over time following an infection; we then in contrast asymptomatic testing methods by calculating the chance {that a} symptomatic an infection is detected earlier than symptom onset and the chance that an asymptomatic an infection is detected inside 7 days of an infection.

Outcomes: We estimated that the chance that the PCR take a look at detected an infection peaked at 77% (54-88%) four days after an infection, reducing to 50% (38-65%) by 10 days after an infection. Our outcomes counsel a considerably greater chance of detecting infections 1-Three days after an infection than beforehand printed estimates. We estimated that testing each different day would detect 57% (33-76%) of symptomatic instances previous to onset and 94% (75-99%) of asymptomatic instances inside 7 days if take a look at outcomes have been returned inside a day.

Conclusions: Our outcomes counsel that routine asymptomatic testing can allow detection of a excessive proportion of contaminated people early of their an infection, offered that the testing is frequent and the time from testing to notification of outcomes is sufficiently quick.

Zintegrowany system Argonaute do PCR z pojedynczą probówką umożliwia bardzo czułe wykrywanie rzadkich mutacji 

Technological advances in uncommon DNA mutations detection have revolutionized the analysis and monitoring of tumors, however they’re nonetheless restricted by the shortage of supersensitive and high-coverage procedures for figuring out low-abundance mutations. Right here, we describe a single-tube, multiplex PCR-based system, A-Star, that includes a hyperthermophilic Argonaute from Pyrococcus furiosus (PfAgo) for extremely environment friendly detection of uncommon mutations useful from its compatibility with DNA polymerase. This novel method makes use of a selected information design technique to permit PfAgo selective cleavage with single-nucleotide decision at 94°C, thus principally eliminating wild-type DNA within the denaturation step and effectively amplifying uncommon mutant DNA in the course of the PCR course of.

The built-in single-tube system achieved nice effectivity for enriching uncommon mutations in contrast with a divided system separating the cleavage and amplification. Thus, A-Star permits simple detection and quantification of 0.01% uncommon mutations with ≥5500-fold improve in effectivity. The feasibility of A-Star was additionally demonstrated for detecting oncogenic mutations in strong tumor tissues and blood samples. Remarkably, A-Star achieved simultaneous detection of a number of oncogenes by a easy single-tube response by orthogonal guide-directed particular cleavage. This examine demonstrates a supersensitive and fast nucleic acid detection system with promising potential for each analysis and therapeutic purposes.

Pacjenci z dodatnim wynikiem testu COVID19-PCR jako negatywna kontrola testów na obecność przeciwciał COVID19 

• COVID-19 serological antibody exams are lately wanted for a comparatively fast, inexpensive, and priceless evaluation of the immunity towards COVID-19 an infection. Moreover, they may also help with evaluating the sufficiency of the vaccination course of and its longevity. There are limitations within the present method of selecting the optimistic and destructive management samples for the validation of these exams.
• Herein, we’re proposing using blood samples from optimistic COVID-19 sufferers, initially of the illness course, as destructive management blood samples for the antibody exams. For extra precision, each the destructive and the optimistic management samples could be obtained from the identical sufferers the place the accuracy of the take a look at will depend upon its means to detect the seroconversion, from destructive to optimistic antibodies detection, inside the identical affected person.
• Moreover, when the validation of the take a look at is accompanied by detecting/sequencing the viral genome in these COVID-19 sufferers, this could additionally help in figuring out the accuracy of the take a look at in detecting the immune response to particular viral variants. The latter notion is required for the right administration of the COVID-19 disaster, new vaccines’ manufacturing, and evaluating the vaccines’ efficiencies. Lastly, this method could possibly be requested/formulated by the regulatory companies as a part of the exams’ validation and could be “in-house” obtained by well being amenities earlier than its scientific use.

Systematyczny przegląd i metaanaliza wypisanych pacjentów COVID-19 z dodatnim wynikiem RT-PCR 

Background: With the elevated variety of sufferers discharged after having COVID-19, increasingly research have reported instances whose retesting was optimistic (RP) throughout the convalescent interval, which brings a brand new public well being problem to the world.

Strategies: We searched PubMed, Net of Science, The Cochrane Library, CNKI, WanFang and VIP from December 1, 2019 to December 31, 2020. The included research have been assessed utilizing JBI vital appraisal instruments and Newcastle-Ottawa Scale. The RP fee of discharge sufferers was analyzed by a meta-analysis. We adhered to PRISMA reporting guideline.

Findings: We’ve got included 117 research with 2669 RP contributors after discharge. The methodological high quality of 66 case studies have been low to excessive, 42 case sequence and three cohort examine have been average to excessive, Three case-control research have been average and three cross-sectional research have been low to average. The scientific manifestations of most RP sufferers have been delicate or asymptomatic, and CT imaging and laboratory examinations have been normally regular. The present danger components counsel that extra consideration needs to be paid to sever sufferers, aged sufferers, and sufferers with co-morbidities. The abstract RP fee was 12·2% (95% CI 10·6-13·7) with excessive heterogeneity (I² = 85%).

Interpretation: To this point, the causes and danger components of RP lead to discharged sufferers usually are not totally understood. Excessive-quality etiological and scientific research are wanted to research these points to additional assist us to make methods to manage and forestall its prevalence.