Strukturalne wglądy w rozpoznawanie metylowanego DNA przez domenę wiążącą metyl-CpG MBD6 z Arabidopsis thaliana

Metylacja cytozyny jest modyfikacją epigenetyczną niezbędną do tworzenia dojrzałej heterochromatyny, wyciszania genów i stabilności genomu. W roślinach metylacja zachodzi nie tylko w obrębie zasad cytozyny w CpG, ale także w kontekstach CpHpG i CpHpH, gdzie H oznacza A, T lub C. Białka z domeną wiążącą metyl-CpG (MBD), które rozpoznają symetryczne dinukleotydy represory genów w komórkach ssaków są również obecne w komórkach roślinnych, chociaż ich właściwości strukturalne i funkcjonalne wciąż pozostają słabo poznane.

Aby wypełnić tę lukę, w tym badaniu określiliśmy strukturę roztworu domeny MBD białka MBD6 z Arabidopsis thaliana i zbadaliśmy jego właściwości wiązania z metylowanym DNA za pomocą testów wiązania i dogłębnej analizy spektroskopowej NMR. Domena AtMBD6 MBD fałduje się w kanoniczną strukturę MBD zgodnie ze swoistością wiązania z metylo-CpG i posiada interfejs wiązania DNA podobny do ssaczych domen MBD.

Co jednak intrygujące, stwierdzono, że powinowactwo wiązania domeny MBD AtMBD6 z DNA zawierającym metylo-CpG jest znacznie niższe niż w przypadku znanych domen MBD ssaków.

Główna różnica wynika z braku dodatnio naładowanych reszt w AtMBD6, które przypuszczalnie oddziałują ze szkieletem DNA, jak widać w ssaczych kompleksach DNA MBD/metylo-CpG. Podsumowując, stworzyliśmy strukturalne podstawy rozpoznawania metylo-CpG przez AtMBD6, aby lepiej zrozumieć, w jaki sposób białka MBD działają jako mediatory sygnałów epigenetycznych w komórkach roślinnych.

Czynniki transkrypcyjne (TF) to białka zaangażowane w regulację ekspresji genów. W epigenetyce ogólnie przyjmuje się, że metylowane nukleotydy mogą zapobiegać wiązaniu TF z fragmentami DNA. Jednak ostatnie badania potwierdziły, że niektóre TF mają zdolność do interakcji z metylowanymi fragmentami DNA w celu dalszej regulacji ekspresji genów.
Chociaż eksperymenty biochemiczne mogą rozpoznać TF wiążące się z metylowanymi sekwencjami DNA, te mokre metody eksperymentalne są czasochłonne i kosztowne. Metody uczenia maszynowego stanowią dobry wybór do szybkiej identyfikacji tych TF bez użycia materiałów eksperymentalnych .
W związku z tym badanie to ma na celu zaprojektowanie solidnego predyktora do wykrywania TF związanych z metylowanym DNA. Najpierw zaproponowaliśmy użycie funkcji wektora słowa tripeptydowego do formułowania próbek białka. Następnie, w oparciu o sieć neuronową rekurencyjną z długotrwałą pamięcią krótkotrwałą, zaprojektowano dwuetapowy model obliczeniowy. Predyktor pierwszego kroku został wykorzystany do odróżnienia czynników transkrypcyjnych od czynników nietranskrypcyjnych.
Gdy białka zostały określone jako TF, zastosowano predyktor drugiego etapu, aby ocenić, czy TF mogą wiązać się z metylowanym DNA . W niezależnym teście zbioru danych dokładność pierwszego i drugiego kroku wynosi odpowiednio 86,63% i 73,59%. Ponadto analiza statystyczna dystrybucji tripeptydów w próbkach treningowych wykazała, że pozycja i liczba niektórych tripeptydów w sekwencji może wpływać na wiązanie TF z metylowanym DNA.
Ostatecznie w oparciu o nasz model powstał darmowy serwer WWW oparty na proponowanym modelu, który jest dostępny pod adresem https://bioinfor.nefu.edu.cn/TFPM/

Metylowane DNA jest nie tylko biomarkerem diagnostycznym, ale także prognostycznym dla wczesnego stadium raka.

Jednak sekwencjonowanie wodorosiarczynem sodu jako „złoty standard” metody wykrywania markerów metylacji ma pewne wady, takie jak czasochłonność i pracochłonność procedur. Dlatego do analizy sekwencji DNA z resztami metylowanymi lub bez nich wymagane są proste i niezawodne metody.

Proponujemy tutaj prostą i bezpośrednią metodę wykrywania metylacji DNA poprzez jego przejście konformacyjne do G-kwadrupleksu przy użyciu tranzystora polowego bramkowanego roztworem (SG-FET) bez użycia materiałów znakowanych. Gen BCL-2 , który bierze udział w rozwoju różnych guzów ludzkich, zawiera segmenty G-bogate i ulega zmianie konformacyjnej do G-kwadrupleksu w zależności od stężenia K ^{+ .}

Skumulowane nici G-kwadrupleksów poruszają się blisko powierzchni czujnika SG-FET, co skutkuje dużymi sygnałami elektrycznymi opartymi na wewnętrznych ładunkach molekularnych. Ponadto gęstą hydrofilową szczoteczkę polimerową zaszczepia się za pomocą inicjowanej powierzchniowo polimeryzacji rodnikowej z przeniesieniem atomu na powierzchnię czujnika SG-FET w celu zmniejszenia szumu elektrycznego w oparciu o niespecyficzną adsorpcję substancji zakłócających.

W szczególności kontrola grubości szczotki polimerowej indukuje sygnały elektryczne w oparciu o ładunki molekularne DNA w warstwie dyfuzyjnej, zgodnie z limitem długości Debye’a. Platforma oparta na czujniku SG-FET z dobrze zdefiniowaną szczoteczką polimerową jest odpowiednia do monitorowania in situ metylowanego DNA i stanowi urządzenie przyłóżkowe o wysokim stosunku sygnału do szumu i bez konieczności stosowania dodatkowych procesów takie jak konwersja wodorosiarczynu i reakcja łańcuchowa polimerazy.

Kilka leków przeciwnowotworowych opiera swoją cytotoksyczność na ich zdolności do interkalowania między parami zasad DNA.

Niemniej jednak ustalono, że mechanizm interkalacji leków w DNA rozpoczyna się od wcześniejszego sposobu interakcji leku z DNA wiązania rowka. Czasami, dla pewnego rodzaju płaskich małych cząsteczek, wiązanie przez rowki nie daje żadnego efektu cytotoksycznego i pożądane jest szybkie przejście takich płaskich małych cząsteczek do trybu interkalacji cytotoksycznej. Tak jest w przypadku metylowanych pochodnych fenantroliny (fen), gdzie zmiany w podstawieniu w pozycji i liczbie grup metylowych decydują o ich zdolności jako związków cytotoksycznych, a zatem jest to sposób na modulację efektów cytotoksycznych.

W tej pracy zbadaliśmy tę modulację za pomocą interakcji kompleksu [Pt(en)(phen)] ²⁺ i kilku pochodnych przez metylację fenu w różnej liczbie i pozycji oraz heksameru DNA d(GTCGAC) ₂przez rowek wiązanie przy użyciu metod PM6-DH2 i DFT-D. Analizę geometrii, struktury elektronowej i energetyki badanych układów porównano z pracami eksperymentalnymi, aby uzyskać wgląd w związek struktura-oddziaływanie dla badanych układów z cytotoksycznością.Trendy są wyjaśnione za pomocą wskaźnika interakcji niekowalencyjnych (NCI), analizy rozkładu energii (EDA) i wkładów solwatacyjnych. Nasze wyniki są zgodne z doświadczeniami, w których metylacja pozycji 4 fenu wydaje się faworyzować interakcję poprzez wiązanie rowka, co utrudnia przejście do interkalacyjnego trybu cytotoksycznego.

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Mouse Telomere Length Quantification qPCR Assay Kit(Relative)
EQ028-100rxns	ELK Biotech	100rxns	125 EUR
Mouse Telomere Length Quantification qPCR Assay Kit(Relative)
EQ028-500rxns	ELK Biotech	500rxns	375 EUR
Human Telomere Length Quantification qPCR Assay Kit(Relative)
EQ022	ELK Biotech	100rxns	375 EUR
Mouse Telomere Length Quantification qPCR Assay Kit(Relative)
EQ028	ELK Biotech	100rxns	375 EUR
Sphingomyelin Quantification Colorimetric Assay Kit
K600-100	Biovision	each	574.8 EUR
VAHTS Library Quantification Kit for Illumina (High ROX Premixed)
NQ104	Vazyme	500 rxn	349.2 EUR
ExoQualiTM Total Exosome Capture & Quantification Kit (Serum)
CEIK-107L-96wells	Creative Bioarray	96 wells	Ask for price
ExoQualiTM Total Exosome Capture & Quantification Kit (Urine)
CEIK-110L-96wells	Creative Bioarray	96 wells	Ask for price
VAHTS Library Quantification Kit for IIIumina (High ROX Premixed)
NQ104-500rxns	Vazyme	500 rxns	181.21 EUR
Sunlong Medical™ Human OSMR ELISA Kit For Protein Quantification
EL0292Hu-48T	Sunlong	48T	398 EUR
Sunlong Medical™ Human OSMR ELISA Kit For Protein Quantification
EL0292Hu-96T	Sunlong	96T	655 EUR
Apoptotic, Necrotic & Healthy Cells Quantification Kit (50 assays)
30018-1	Biotium	KT	242 EUR
HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit
K2122-100	ApexBio	100 assays	667 EUR
HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit
K613-100	Biovision	each	783.6 EUR
ExoQualiTM Total Exosome Capture & Quantification Kit (Plasma)
CEIK-108L-96wells	Creative Bioarray	96 wells	Ask for price
Sunlong Medical™ Human ACBD6 ELISA Kit For Protein Quantification
EL0303Hu-48T	Sunlong	48T	398 EUR
Sunlong Medical™ Human ACBD6 ELISA Kit For Protein Quantification
EL0303Hu-96T	Sunlong	96T	Ask for price
QuantiSir Specific Gene Knockdown Quantification Kit For Cell Cycle Regulation - TS
P-5005	EpiGentek	96 Assays	945.66 EUR
Detergent Compatible Bradford Protein Quantification Kit
E211-01	Vazyme	500 rxn	213.6 EUR
EpiQuik 8-OhG RNA Damage Quantification Direct Kit (Colorimetric)
P-6008	EpiGentek	48 Assays	640 EUR
MycoLightâ„¢ Fluorimetric CTC Live Bacteria Quantification Kit
22405-100Tests	AAT Bioquest	100 Tests	334 EUR
Free Fatty Acid Quantification Colorimetric/Fluorometric Kit
K2121-100	ApexBio	100 assays	624 EUR
Free Fatty Acid Quantification Colorimetric/Fluorometric Kit
K612-100	Biovision	each	732 EUR
ExoTEST™ for Overall Exosome capture and quantification from Cell media
HBM-RTK-POC/TC	HansaBioMed	KIT	506.52 EUR
QuantiSir Specific Gene Knockdown Quantification Kit For Cell Death/Apoptosis - XIAP
P-5004	EpiGentek	96 Assays	945.66 EUR
QuantiSir Specific Gene Knockdown Quantification Kit For Cell Proliferation - Wnt 4
P-5006	EpiGentek	96 Assays	945.66 EUR
EpiQuik Methylated DNA Immunoprecipitation Kit
P-2019	EpiGentek	Ask for price Ask for price Ask for price	48 Reactions 24 Reactions 48 Reactions
QuantiSir Specific Gene Knockdown Quantification Kit For Signal Transduction - ViDR
P-5008	EpiGentek	96 Assays	945.66 EUR
QuantiSir Specific Gene Knockdown Quantification Kit For Transcription Factor - YY-1
P-5009	EpiGentek	96 Assays	945.66 EUR
Triglyceride Quantification Colorimetric/Fluorometric Kit
K2127-100	ApexBio	100 assays	629 EUR
RNase Activity Detection/Quantification Assay Kit (Fluorometric)
K934-100	Biovision	each	574.8 EUR
Triglyceride Quantification Colorimetric/Fluorometric Kit
K622-100	Biovision	each	744 EUR
EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric)
P-4010	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Fluorometric)
P-4011	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H4K5 Quantification Kit (Colorimetric)
P-4022	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H4K5 Quantification Kit (Fluorometric)
P-4023	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H4K8 Quantification Kit (Colorimetric)
P-4024	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H4K8 Quantification Kit (Fluorometric)
P-4025	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
ExoTEST™ for Overall Exosome capture and quantification from human plasma
HBM-RTK-POF/TP	HansaBioMed	KIT	506.52 EUR
ExoTEST™ for Overall Exosome capture and quantification from human urine
HBM-RTK-POF/TU	HansaBioMed	KIT	506.52 EUR
ExoTEST™ for Overall Exosome capture and quantification from human serum
HBM-RTK-POS/TS	HansaBioMed	KIT	506.52 EUR
QuantiSir Specific Gene Knockdown Quantification Kit For Epigenetic Regulators - TFIIB
P-5002	EpiGentek	96 Assays	945.66 EUR
QuantiSir Specific Gene Knockdown Quantification Kit For Tumor Suppressor/Oncogene - WT-1
P-5007	EpiGentek	96 Assays	945.66 EUR
EpiQuik Global Acetyl Histone H4K16 Quantification Kit (Fluorometric)
P-4027	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H4K12 Quantification Kit (Colorimetric)
P-4028	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H4K12 Quantification Kit (Fluorometric)
P-4029	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric)
P-4059	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K4 Quantification Kit (Colorimetric)
P-3022	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K4 Quantification Kit (Fluorometric)
P-3023	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K9 Quantification Kit (Colorimetric)
P-3032	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K9 Quantification Kit (Fluorometric)
P-3033	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H4R3 Quantification Kit (Colorimetric)
P-3090	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Circulating Total Histone H3 Quantification Kit (Colorimetric)
P-3091	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3R8 Quantification Kit (Colorimetric)
P-3096	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Colorimetric)
P-4012	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Fluorometric)
P-4013	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Colorimetric)
P-4014	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Fluorometric)
P-4015	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K23 Quantification Kit (Colorimetric)
P-4016	EpiGentek	96 Assays	945.66 EUR
EpiQuik Global Acetyl Histone H3K23 Quantification Kit (Fluorometric)
P-4017	EpiGentek	96 Assays	945.66 EUR
EpiQuik Global Acetyl Histone H3K36 Quantification Kit (Colorimetric)
P-4018	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K36 Quantification Kit (Fluorometric)
P-4019	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K56 Quantification Kit (Colorimetric)
P-4020	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H3K56 Quantification Kit (Fluorometric)
P-4021	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Acetyl Histone H4K16 Quantification Kit (Colorimetric)
P-4026	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
ExoQualiTM Total Exosome Capture & Quantification Kit (Cell Supernatant)
CEIK-109L-96wells	Creative Bioarray	96 wells	Ask for price
Fully CpG Methylated Human Genomic DNA
X10000	EpiGentek	10 µg/100 µl	Ask for price
ExoELISA-Step for detection and quantification of exosomes from cell culture
Exo2506	ImmunoStep	96	654.5 EUR
EZScreen? Triglyceride Quantification Colorimetric Assay Kit (384-well)
K952-400	Biovision	each	751.2 EUR
EpiQuik Global Mono-Methyl Histone H3K4 Quantification Kit (Colorimetric)
P-3024	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Mono-Methyl Histone H3K4 Quantification Kit (Fluorometric)
P-3025	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K4 Quantification Kit (Colorimetric)
P-3026	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K4 Quantification Kit (Fluorometric)
P-3027	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K4 Quantification Kit (Colorimetric)
P-3028	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K4 Quantification Kit (Fluorometric)
P-3029	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Mono-Methyl Histone H3K9 Quantification Kit (Colorimetric)
P-3030	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Mono-Methyl Histone H3K9 Quantification Kit (Fluorometric)
P-3031	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K9 Quantification Kit (Colorimetric)
P-3034	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K9 Quantification Kit (Fluorometric)
P-3035	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K9 Quantification Kit (Colorimetric)
P-3036	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K9 Quantification Kit (Fluorometric)
P-3037	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K27 Quantification Kit (Colorimetric)
P-3040	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K27 Quantification Kit (Fluorometric)
P-3041	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K27 Quantification Kit (Colorimetric)
P-3042	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K27 Quantification Kit (Fluorometric)
P-3043	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K27 Quantification Kit (Colorimetric)
P-3044	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K27 Quantification Kit (Fluorometric)
P-3045	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K36 Quantification Kit (Colorimetric)
P-3048	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K36 Quantification Kit (Fluorometric)
P-3049	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K36 Quantification Kit (Colorimetric)
P-3050	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K36 Quantification Kit (Fluorometric)
P-3051	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K36 Quantification Kit (Colorimetric)
P-3052	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K36 Quantification Kit (Fluorometric)
P-3053	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K79 Quantification Kit (Colorimetric)
P-3056	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H3K79 Quantification Kit (Fluorometric)
P-3057	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K79 Quantification Kit (Colorimetric)
P-3058	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H3K79 Quantification Kit (Fluorometric)
P-3059	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K79 Quantification Kit (Colorimetric)
P-3060	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H3K79 Quantification Kit (Fluorometric)
P-3061	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H4K20 Quantification Kit (Colorimetric)
P-3066	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Di-Methyl Histone H4K20 Quantification Kit (Fluorometric)
P-3067	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H4K20 Quantification Kit (Colorimetric)
P-3068	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Tri-Methyl Histone H4K20 Quantification Kit (Fluorometric)
P-3069	EpiGentek	Ask for price Ask for price Ask for price	96 Assays 48 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H4K20 Quantification Kit (Colorimetric)
P-3070	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays
EpiQuik Global Pan-Methyl Histone H4K20 Quantification Kit (Fluorometric)
P-3071	EpiGentek	Ask for price Ask for price	96 Assays 96 Assays

Patrząc na wyniki NCI, oddziaływania te pochodzą nie tylko z oddziaływań CH/π i CH/n grupy metylowej w pozycji 4, ale także z ligandu etylenodiaminy (en), którego orientację w kompleksie Pt stwierdzono w ten sposób że wytwarza dużą liczbę słabych interakcji z DNA, zwłaszcza ze szkieletem cukrowym i fosforanowym